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人VCAM1 ELISA试剂盒(CD106) (ab46118)

Reviews (1)Q&A (1)References (3)
Typical Standard Curve

    Key features and details

    • Sensitivity: 0.6 ng/ml
    • Range: 1.56 ng/ml - 50 ng/ml
    • Sample type: Cell culture supernatant, Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

    概述

    • 产品名称

      人VCAM1 ELISA试剂盒(CD106)
      参阅全部 VCAM1 试剂盒

    • 检测方法

      Colorimetric

    • 精确度

      批次内
      样品 n Mean SD CV%
      A 8 48.79ng/ml 0.221 0.45%
      B 8 5.81ng/ml 0.132 2.27%
      批次间
      样品 n Mean SD CV%
      A 28 49.69ng/ml 0.72 1.44%
      B 28 5.95ng/ml 0.354 5.94%

    • 样品类型

      Cell culture supernatant, Serum, Plasma

    • 检测类型

      Sandwich (quantitative)

    • 灵敏度

      < 0.6 ng/ml

    • 范围

      1.56 ng/ml - 50 ng/ml

    • 检测时间

      1h 40m

    • 实验步骤

      Multiple steps standard assay

    • 种属反应性

      与反应: Human

    • 产品概述

      Abcam’s Human VCAM1 (CD106) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of VCAM1 in cell culture supernatants, buffered solutions, serum, plasma, and other body fluids.


      A monoclonal antibody specific for VCAM1 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known VCAM1 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for VCAM1 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of VCAM1 present in the samples.


      This kit will recognize both endogenous and recombinant Human VCAM1.


      Get results in 90 minutes with Human VCAM1 ELISA Kit (CD106) (ab187393) from our SimpleStep ELISA® range.


       

    • 平台

      Microplate

    性能

    图片

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab46118.

    文献 (3)

    发表研究结果有使用 ab46118？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab46118 被引用在 3 文献中.

    • Ounjaijean S  et al. Cardiovascular risks in Asian HIV-infected patients receiving boosted-protease inhibitor-based antiretroviral treatment. J Infect Dev Ctries 15:289-296 (2021). PubMed: 33690213
    • Sun Q  et al. A Allele of ICAM-1 Rs5498 and VCAM-1 Rs3181092 is Correlated with Increased Risk for Periodontal Disease. Open Life Sci 14:638-646 (2019). PubMed: 33817202
    • Harling L  et al. Pre-operative serum VCAM-1 as a biomarker of atrial fibrillation after coronary artery bypass grafting. J Cardiothorac Surg 12:70 (2017). PubMed: 28821262

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    1-2 of 2 Abreviews or Q&A

    VCAM1 concentration in human plasma

     Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    abreview image
    Human plasma samples were diluted 50 times by diluent buffer.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jun 18 2013

    Question

    What formula do you recommend for creating the standard curve?

    Read More
    Answer

    Thank you for contacting us. The laboratory that developed and validated the kit uses linear regression but there are some who argue that 4 parameter logisitic nonlinear regression is a better way to curve-fit ELISA data. Either formula is applicable for this kit. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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