Key features and details
- Sensitivity: 70 pg/ml
- Range: 0.069 ng/ml - 50 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
样品类型Cell culture supernatant, Serum, Plasma
灵敏度< 70 pg/ml
范围0.069 ng/ml - 50 ng/ml
> 100 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 124.4 106% - 134% Serum 125.5 91% - 145% Plasma 116 94% - 115%
实验步骤Multiple steps standard assay
Abcam’s TREM1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TREM1 in serum, plasma and cell culture supernatants. (Human TREM1 concentration is pretty low in normal serum, it may not be detected in this assay).
This assay employs an antibody specific for Human TREM1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TREM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TREM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TREM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Human soluble TREM1 ELISA Kit (ab185987) from our SimpleStep ELISA® range.
Optimization may be required with urine samples.
存放说明Store at -20°C. Please refer to protocols.
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 500X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Human TREM1 2 vials Recombinant Human TREM1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml TREM1 Microplate (12 x 8 wells) 1 unit
相关性TREM1 is also known as triggering receptor expressed on myeloid cells 1, and triggering receptor expressed on monocytes 1. TREM1 is a Type I membrane protein that contains an immunoglobulin-like V-type domain. An alternatively spliced protein variant may be secreted. TREM1 is highly expressed on peripheral blood myeloid cells (particularly mature monocytes and granulocytes); TREM1 expression can be further upregulated by bacteria, fungi and lipopolysaccharide. TREM1 has been shown to interact with the adaptor protein DAP12 to stimulate neutrophil and monocyte-mediated inflammatory responses through the triggering and release of pro-inflammatory cytokines and chemokines. TREM1 is thought to amplify inflammatory responses to fungal and bacterial infections and potentiate septic shock.
细胞定位Secreted and Cell membrane.
- TREM 1
- Triggering receptor expressed on monocytes 1
ab100659 被引用在 1 文献中.
- Arízaga-Ballesteros V et al. Can sTREM-1 predict septic shock & death in late-onset neonatal sepsis? A pilot study. Int J Infect Dis 30:27-32 (2015). PubMed: 25461656