人TNF alpha ELISA试剂盒(ab46087)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 25 pg/ml - 800 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人TNF alpha ELISA试剂盒
参阅全部 TNF alpha 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Sample 6 3.3% 批次间 样品 n Mean SD CV% Sample 6 9% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
= 10 pg/ml -
范围
25 pg/ml - 800 pg/ml -
回收率
107 %
特定样本回收率 样品类型 平均% 范围 Serum 81 74% - 90% -
检测时间
3h 40m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Human TNF alpha ELISA kit (ab46087) is a quantitative ELISA assay for the measurement of TNF alpha in supernatants, buffered solutions, serum and plasma samples.
A monoclonal antibody specific TNF alpha has been coated onto the wells of the microtiter strips provided. Samples, including standards of known TNF alpha concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for TNF alpha are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of TNF alpha present in the samples.
This kit will recognize both endogenous and recombinant Human TNF alpha.
Get results in 90 minutes with Human TNF alpha ELISA Kit (ab181421) from our SimpleStep ELISA® range.
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 1 x 96 tests 2 x 96 tests 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 1 x 15ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml 1 x 7.5ml 1 x 13ml Biotinylated anti-TNF alpha Red 1 x 400µl 2 x 400µl 1 x 400µl 2 x 400µl Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml 1 x 11ml 1 x 24ml HRP Diluent Red 1 x 12ml 1 x 23ml 1 x 12ml 1 x 23ml Plastic Plate Covers 1 x 2 units 1 x 4 units 1 x 2 units 1 x 4 units Standard Diluent (Serum) 1 x 7ml 2 x 7ml 1 x 7ml 2 x 7ml Stop Reagent Black 1 x 11ml 2 x 11ml 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl 2 x 5µl 4 x 5µl TNF alpha Control Yellow 2 vials 4 vials 2 vials 4 vials TNF alpha Microplate (12 x 8 well strips) 1 unit 2 units 1 unit 2 units TNF alpha Standard 2 vials 4 vials 2 vials 4 vials -
研究领域
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功能
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. -
疾病相关
Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). -
序列相似性
Belongs to the tumor necrosis factor family. -
翻译后修饰
The soluble form derives from the membrane form by proteolytic processing.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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别名
- APC1
- APC1 protein
- Cachectin
see all -
数据库链接
- Entrez Gene: 7124 Human
- Omim: 191160 Human
- SwissProt: P01375 Human
- Unigene: 241570 Human
相关产品
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Positive Controls
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SimpleStep ELISA kits
图片
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Typical Standard Curve
Representative standard curve using ab46087
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Sandwich ELISA - TNF alpha Human ELISA Kit (ab46087)TNF? was measured in cell culture medium from U937 control cells, or cells stimulated with LPS (1 µg x mL-1; 6 h) or PMA (10 ng x mL-1; 24 h) with LPS and control and stimulated PBMCs (PHA 10 µg x mL-1; 3 days). Results are shown with background signal subtracted (duplicates +/- SD), with the positive control from ab46087 included.
数据表及文件
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SDS download
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Datasheet download
文献 (27)
ab46087 被引用在 27 文献中.
- Zhang G et al. Exosomes from HPV-16 E7-pulsed dendritic cells prevent the migration, M1 polarization, and inflammation of macrophages in cervical cancer by regulating catalase 2 (CAT2). Ann Transl Med 10:217 (2022). PubMed: 35280390
- Nobile V et al. Antioxidant Efficacy of a Standardized Red Orange (Citrus sinensis (L.) Osbeck) Extract in Elderly Subjects: A Randomized, Double Blind, Controlled Study. Nutrients 14:N/A (2022). PubMed: 36296919
- Baruah V et al. Prognostic, clinical, and therapeutic importance of RANTES-CCR5 axis in hepatitis A infection: A multiapproach study. J Med Virol 93:3656-3665 (2021). PubMed: 32975838
- Saleem U et al. Pharmacological Screening of Viola odorata L. for Memory-Enhancing Effect via Modulation of Oxidative Stress and Inflammatory Biomarkers. Front Pharmacol 12:664832 (2021). PubMed: 34149418
- Chen L et al. Upregulating miR-27a-3p inhibits cell proliferation and inflammation of rheumatoid arthritis synovial fibroblasts through targeting toll-like receptor 5. Exp Ther Med 22:1227 (2021). PubMed: 34539823