人Thyroid Stimulating Hormone ELISA试剂盒(TSH) (ab100660)
Key features and details
- Sensitivity: 4 pg/ml
- Range: 2.744 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Thyroid Stimulating Hormone ELISA试剂盒(TSH) -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 4 pg/ml -
范围
2.744 pg/ml - 2000 pg/ml -
回收率
86 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 82.76 70% - 90% Serum 97.89 75% - 120% Plasma 78.59 67% - 86% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s TSH Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TSH in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human TSH coated on a 96-well plate. Standards and samples are pipetted into the wells and TSH present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TSH antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TSH bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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说明
Optimization may be required with urine samples.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human TSH 2 vials Recombinant Human TSH Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml TSH Microplate (12 x 8 wells) 1 unit -
研究领域
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相关性
Thyroid stimulating hormone, also known as thyrotropin, is secreted from cells in the anterior pituitary called thyrotrophs, finds its receptors on epithelial cells in the thyroid gland, and stimulates that gland to synthesize and release thyroid hormones. TSH is a glycoprotein hormone composed of two subunits which are non covalently bound to one another. The alpha subunit of TSH is also present in two other pituitary glycoprotein hormones, follicle stimulating hormone and luteinizing hormone, and, in primates, in the placental hormone chorionic gonadotropin. Each of these hormones also has a unique beta subunit, which provides receptor specificity. In other words, TSH is composed of alpha subunit bound to the TSH beta subunit, and TSH associates only with its own receptor. Free alpha and beta subunits have essentially no biological activity. -
细胞定位
Secreted -
别名
- Anterior pituitary glycoprotein hormones common subunit alpha
- CG ALPHA
- CGA
see all -
数据库链接
- Entrez Gene: 1081 Human
- Omim: 118850 Human
- SwissProt: P01215 Human
图片
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Typical standard curve
Representative standard curve using ab100660
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Typical standard curveRepresentative standard curve using ab100660
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab100660 被引用在 1 文献中.
- Wilcox MD et al. A Pilot Pre and Post 4 Week Intervention Evaluating the Effect of a Proprietary, Powdered, Plant Based Food on Micronutrient Status, Dietary Intake, and Markers of Health in a Healthy Adult Population. Front Nutr 9:945622 (2022). PubMed: 35903454