人P cadherin ELISA试剂盒(ab100621)
Key features and details
- Sensitivity: 20 pg/ml
- Range: 2.47 pg/ml - 18000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人P cadherin ELISA试剂盒
参阅全部 P cadherin 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 20 pg/ml -
范围
2.47 pg/ml - 18000 pg/ml -
回收率
> 100 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 129.1 117% - 138% Serum 121.3 111% - 131% Plasma 114.9 105% - 125% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s P cadherin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human P cadherin in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human P cadherin coated on a 96-well plate. Standards and samples are pipetted into the wells and P cadherin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human P cadherin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of P cadherin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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说明
Optimization may be required with urine samples
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Human P cadherin 2 vials P cadherin Microplate (12 x 8 wells) 1 unit Recombinant Human P cadherin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. -
组织特异性
Expressed in some normal epithelial tissues and in some carcinoma cell lines. -
疾病相关
Defects in CDH3 are the cause of hypotrichosis with juvenile macular dystrophy (HJMD) [MIM:601553]. HJMD is a rare autosomal recessive disorder characterized by early hair loss heralding severe degenerative changes of the retinal macula and culminating in blindness during the second to third decade of life.
Defects in CDH3 are the cause of ectodermal dysplasia with ectrodactyly and macular dystrophy (EEM) [MIM:225280]; also known as EEM syndrome, Albrectsen-Svendsen syndrome or Ohdo-Hirayama-Terawaki syndrome. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EEM is an autosomal recessive condition characterized by features of ectodermal dysplasia such as sparse eyebrows and scalp hair, and selective tooth agenesis associated with macular dystrophy and ectrodactyly. -
序列相似性
Contains 5 cadherin domains. -
细胞定位
Cell membrane. - Information by UniProt
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别名
- CADH3_HUMAN
- Cadherin 3
- Cadherin 3 precursor
see all -
数据库链接
- Entrez Gene: 1001 Human
- Omim: 114021 Human
- SwissProt: P22223 Human
- Unigene: 191842 Human
图片
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Typical Standard Curve
Representative Standard Curve using ab100621.
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Typical Standard CurveRepresentative Standard Curve using ab100621.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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