人MCP1 ELISA试剂盒(ab100586)

Please note this product is unpublished so when you decide to order you need to contact our customer service team for them to put this item in your order.

I hope this info will help. Please let me know if you experience any difficulties.

This product is available but not published on our website. You cannot purchase this using our normal ordering system however I can add this product to your next shipment. Could you confirm how many vials customer would need..6?.
In my last I made an error in calculating the price; the price would be $720 for 6 vials.

The concentration of HRP-streptavidin is not important. The 200ul solution could be used as recommended in protocol. Please note customer have to use new protocol with these new vials, which is available on our website. Each kit protocol have the information on how to use the 200ul vial of HRP-Strep conjugate.

I hope this information will be helpful.

Thank you for contacting us. I apologize for the delay.

We are ready to publish this product. The product would be available at US dollar 120 per 200ul. The customer would need 6 vials of product so it will cost them $720 excluding shipping charges. Could you please let us know if customer would like to purchase the product?
Looking forward to hearing from you soon.

Thank you for your email. I am sorry for the delay while I was in contact with lab.

The volume of HRP-Streptavidin is very small and therefore would be difficult to see with the naked eye. The HRP-streptavidin volume sent was 8ul and is very concentrated so it is especially important that the vial is spun down before opening to make sure no solution is adhering to the inside walls or cap of the vial to ensure complete recovery. Please have your researchers perform this preparation and they should be able to use the 8 ul HRP. Below are some additional tips for this component:

HRP-Streptavidin – comes as a liquid concentrate. Before withdrawing any liquid from the tube, be sure to centrifuge the vial and pipette up and down to mix thoroughly, as precipitation may form in storage. Once diluted, the HRP-strep must be used up that day. Do not store.

I am also in contact with lab regarding the diluents need to be used for diluting the HRP-Streptavidin vial. I will get back to you with the information soon.

Please note, the expiration date of kit is 6 month from date of purchase including HRP-Streptavidin. Once the HRP streptavidin is reconstituted it should be used same day.

Finally, I can suggest using one kit with the provided HRP-Streptavidin, If in case it does not work then you may buy HRP- streptavidin separately. I will confirm the price if you are interested in buying this.

I hope this information will be helpful. I will contact you with more information soon.

Thank you for contacting us. Here is the requested information: ab46087: Normal values for TNFa in such samples are negatives or very low, so we usually recommend to assay the human serum samples neat with Standard buffer, human serum to dilute the standard curve, or at the 1:2 dilution with standard buffer diluent PBS BSA (in that case, the standard curve should be diluted in same buffer). Below are our recommended normal human serum dilutions for the other 3 kits. These recommendations should only be used as a starting point and the optimal sample dilution needs to be determined by the enduser empirically based on researched literature and knowledge of their particular sample. ab100586: 2 fold dilution ab100610: 500-2000 fold dilution ab100640: 5-200 fold dilution I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for your inquiry.  Below are the plasma values that we have measure during our QC tests with the mentioned ELISA kits: ab100575 : Ranging from undetectable to ~100pg/ml ab100586 : Ranging from undetectable to ~400pg/ml I hope this information helps.  Please let me know if you have any questions or comments.  Thanks!    

Thank you for contacting us. Unfortunately the ELISA assays have not been used in conjunction with a DSX analyser (and we therefore do not have any specific protocols for this) but as long as the machine can accurately carry out the protocol as stated in the manual we can see no reason why it would not be compatible. The "cover well" directions prior to incubation are there to avoid contamination and evaporation of the reagents. The only incubation that is light-sensitive is the TMB incubation. Additionally we recommend performing the incubations under gentle shaking/rocking (regardless of temperature or time). I hope this information has been helpful to you. If you have any further questions please do not hesitate to contact us.