Key features and details
- Assay type: Sandwich (qualitative)
- Sample type: Suspension cells
- Reacts with: Human
参阅全部 Interferon gamma 试剂盒
实验步骤Multiple steps standard assay
Abcam’s Human Interferon gamma ELISPOT Set is an in vitro ELISPOT assay designed for the qualitative measurement of IFNγ production and secretion in a single cell suspension.
The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. ELISPOT assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.
This ELISPOT assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.
After cell stimulation, locally produced cytokines are captured by a specific monoclonal antibody. After cell lysis, trapped cytokine molecules are revealed by a secondary biotinylated detection antibody, which is in turn recognised by streptavidin conjugated to alkaline phosphatase. PVDF-bottomed-well plates are then incubated with BCIP/NBT substrate. Colored "purple" spots indicate cytokine production by individual cells.
Recognizes natural human Interferon Gamma.
经测试应用适用于: ELISpotmore details
存放说明Store at +4°C. Please refer to protocols.
组件 5 x 96 tests 10 x 96 tests 15 x 96 tests 20 x 96 tests Biotinylated Human IFNγ Detection Antibody 1 vial 2 vials 3 vials 4 vials Bovine Serum Albumin 1 x 2g 2 x 2g 3 x 2g 4 x 2g Human IFN Gamma Capture antibody 1 x 0.5ml 2 x 0.5ml 3 x 0.5ml 4 x 0.5ml Ready-to-use BCIP/NBT substrate buffer 1 x 50ml 2 x 50ml 3 x 50ml 8 x 25ml Streptavidin - Alkaline Phosphatase conjugated 1 x 50µl 2 x 50µl 3 x 50µl 4 x 50µl
功能Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
组织特异性Released primarily from activated T lymphocytes.
疾病相关In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
序列相似性Belongs to the type II (or gamma) interferon family.
翻译后修饰Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
- Information by UniProt
- IF 1
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Use at an assay dependent dilution.
Use at an assay dependent dilution.