人Interferon Gamma + IL2 FLUOROSPOT试剂盒(ab48452)
Key features and details
- Assay type: Sandwich (qualitative)
- Detection method: Fluorescent
- Sample type: Suspension cells
- Reacts with: Human
概述
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产品名称
人Interferon Gamma + IL2 FLUOROSPOT试剂盒
参阅全部 Interferon Gamma + IL2 试剂盒 -
检测方法
Fluorescent -
样品类型
Suspension cells -
检测类型
Sandwich (qualitative) -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, oncology, infectious diseases, autoimmune diseases and transplantation.
This Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.
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经测试应用
适用于: FluroSpotmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 5 x 96 tests 10 x 96 tests 1 x 96 tests Anti-FITC antibody green fluorescence conjugate. 1 vial 1 vial 1 vial Biotinylated detection antibody for IL-2 (resuspend in 0.55mL). 1 vial 2 vials 1 vial Bovine Serum Albumin 1 x 2g 2 x 2g 1 x 2g FITC conjugated detection antibody for IFNg (resuspend in 0.55mL) 1 vial 2 vials 1 vial Human IFN Gamma Capture antibody 1 x 500µl 2 x 500µl 1 x 100µl Human IL-2 Capture antibody 1 x 500µl 2 x 500µl 1 x 100µl Streptavidin-phycoerythrin conjugate. 1 vial 1 vial 1 vial -
研究领域
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相关性
IFN-gamma is produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. -
细胞定位
Secreted -
别名
- IFN gamma
- IFNG
- IL2
see all -
数据库链接
- Entrez Gene: 3458 Human
- Entrez Gene: 3558 Human
- SwissProt: P01579 Human
- SwissProt: P60568 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab48452于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| FluroSpot |
Use at an assay dependent dilution.
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| 说明 |
|---|
|
FluroSpot
Use at an assay dependent dilution. |
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab48452 被引用在 1 文献中.
- Kuloğlu ZE et al. Effect of BTN162b2 and CoronaVac boosters on humoral and cellular immunity of individuals previously fully vaccinated with CoronaVac against SARS-CoV-2: A longitudinal study. Allergy 77:2459-2467 (2022). PubMed: 35437772