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人IL-8 ELISA试剂盒(ab46032)

Reviews (1)Q&A (5)References (62)
Sandwich ELISA - IL-8 Human ELISA Kit (ab46032)
  • Typical Standard Curve

Key features and details

  • Sensitivity: 12.3 pg/ml
  • Range: 31.25 pg/ml - 1000 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

概述

  • 产品名称

    人IL-8 ELISA试剂盒
    参阅全部 IL-8 试剂盒

  • 检测方法

    Colorimetric

  • 精确度

    批次内
    样品 n Mean SD CV%
    Pooled Data 6 4%
    批次间
    样品 n Mean SD CV%
    Pooled Data 6 6%

  • 样品类型

    Cell culture supernatant, Serum, Plasma

  • 检测类型

    Sandwich (quantitative)

  • 灵敏度

    < 12.3 pg/ml

  • 范围

    31.25 pg/ml - 1000 pg/ml

  • 回收率

    88 %

  • 检测时间

    2h 15m

  • 实验步骤

    Multiple steps standard assay

  • 种属反应性

    与反应: Human

  • 产品概述

    Human IL-8 ELISA kit is designed for the quantitative measurement of IL-8 supernatants, buffered solutions, serum and plasma samples. (Genecards: CXCL8)


    A monoclonal antibody specific for IL-8 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-8 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-8 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-8 present in the samples.


    This kit will recognize both endogenous and recombinant Human IL-8.

  • 平台

    Microplate

性能

  • 存放说明

    Store at +4°C. Please refer to protocols.
  • 组件 标识符 2 x 96 tests 1 x 96 tests 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 15ml 1 x 15ml 1 x 25ml
    200X Wash Buffer White 2 x 10ml 1 x 10ml 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 13ml 1 x 7.5ml 1 x 7.5ml 1 x 13ml
    Biotinylated anti-IL-8 Red 2 x 0.4ml 1 x 0.4ml 1 x 0.4ml 2 x 0.4ml
    Chromogen TMB Substrate Solution 1 x 24ml 1 x 11ml 1 x 11ml 1 x 24ml
    HRP Diluent Red 1 x 23ml 1 x 12ml 1 x 12ml 1 x 23ml
    IL-8 Control Silver 4 vials 2 vials 2 vials 4 vials
    IL-8 Microplate (12 x 8 well strips) 2 units 1 unit 1 unit 2 units
    IL-8 Standard Yellow 4 vials 2 vials 2 vials 4 vials
    Plastic Plate Covers 4 units 2 units 2 units 4 units
    Standard Diluent (Serum) 2 x 7ml 1 x 7ml 1 x 7ml 2 x 7ml
    Stop Reagent Black 2 x 11ml 1 x 11ml 1 x 11ml 2 x 11ml
    Streptavidin-HRP 4 x 5µl 2 x 5µl 2 x 5µl 4 x 5µl

  • 研究领域

  • 功能

    IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.

  • 序列相似性

    Belongs to the intercrine alpha (chemokine CxC) family.

  • 翻译后修饰

    Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.

  • 细胞定位

    Secreted.
  • Information by UniProt

  • 别名

    • (Ala-IL-8)77
    • (Ser-IL-8)72
    • 9E3
    • Beta thromboglobulin like protein
    • C-X-C motif chemokine 8
    • CEF-4
    • chemokine, CXC motif, ligand 8
    • CXCL8
    • Emoctakin
    • GCP-1
    • GCP/IL-8 protein I
    • GCP/IL-8 protein II
    • GCP/IL-8 protein III
    • GCP/IL-8 protein IV
    • GCP/IL-8 protein V
    • GCP/IL-8 protein VI
    • GCP1
    • Granulocyte chemotactic protein 1
    • IL-8
    • IL-8(1-77)
    • IL-8(9-77)
    • IL8
    • IL8/NAP1 form I
    • IL8/NAP1 form II
    • IL8/NAP1 form III
    • IL8/NAP1 form IV
    • IL8/NAP1 form V
    • IL8/NAP1 form VI
    • IL8_HUMAN
    • Inteleukin 8
    • LECT
    • LUCT
    • Lymphocyte-derived neutrophil-activating factor
    • LYNAP
    • MDNCF
    • MDNCF-b
    • MDNCF-c
    • MONAP
    • Monocyte derived neutrophil activating peptide
    • Monocyte derived neutrophil chemotactic factor
    • Monocyte-derived neutrophil chemotactic factor
    • Monocyte-derived neutrophil-activating peptide
    • NAF
    • NAP 1
    • NAP-1
    • NAP1
    • Neutrophil activating peptide 1
    • Neutrophil activating protein 1
    • Neutrophil-activating factor
    • Neutrophil-activating protein 1
    • Protein 3 10C
    • Protein 3-10C
    • SCYB 8
    • SCYB8
    • Small inducible cytokine subfamily B member 8
    • T cell chemotactic factor
    • T-cell chemotactic factor
    • TSG 1
    • TSG1
    see all

  • 数据库链接

    图片

    • Sandwich ELISA - IL-8 Human ELISA Kit (ab46032)
      Sandwich ELISA - IL-8 Human ELISA Kit (ab46032)
      IL-8 detected in supernatants from control cells (C) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) (P), and with the addition of 1 ug x mL-1 of LPS (Sigma) (P/L) for the last 6 hours. The positive control is included (Pos.). Results shown after background signal was subtracted (duplicates +/- SD).
    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab46032

    文献 (62)

    发表研究结果有使用 ab46032？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab46032 被引用在 62 文献中.

    • Tan CYR  et al. Nicotinamide Prevents UVB- and Oxidative Stress‒Induced Photoaging in Human Primary Keratinocytes. J Invest Dermatol 142:1670-1681.e12 (2022). PubMed: 34740582
    • Niu F  et al. CircRNA circFADS2 is under-expressed in sepsis and protects lung cells from LPS-induced apoptosis by downregulating miR-133a. J Inflamm (Lond) 19:4 (2022). PubMed: 35279129
    • Li L  et al. Transient Receptor Potential Cation Channel Subfamily V Member 1 Expression Promotes Chemoresistance in Non-Small-Cell Lung Cancer. Front Oncol 12:773654 (2022). PubMed: 35402237
    • Yang S  et al. Antioxidant Activities and Mechanisms of Tomentosin in Human Keratinocytes. Antioxidants (Basel) 11:N/A (2022). PubMed: 35624854
    • Gong Y  et al. RAB11A aggravates PDGF-BB-stimulated proliferation, migration, and inflammation of airway smooth muscle cells via affecting the NF-κB and PI3K/AKT pathways. Allergol Immunopathol (Madr) 50:147-154 (2022). PubMed: 35789415
    View all Publications for this product

    客户评价及客户问答

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    1-6 of 6 Abreviews or Q&A

    Abreview for IL-8 ELISA Kit

     Good Excellent 5/5 (Ease of Use)
    Abreviews
    abreview image
    This kit was used to measure the level of IL-8 secretion in to the medium after infecting cells with E.coli strains. The kit was nicely sensitive to detect the level of IL-8 in the medium and was very helpful.
    The standards and the control vial worked very nicely and gave a nice curve.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Aug 13 2019

    Question

    Pourriez-vous svp me dire, pour chaque référence, s’il est possible de modifier la gamme de standards.

    C’est-à-dire, pour votre kit IL8 le standard est reconstitué pour obtenir du 2.000 pg/mL, point le plus haut de la gamme.

    Est-il possible de le reconstituer dans un plus petit volume, pour obtenir par exemple du 4.000 pg/mL et ainsi pouvoir commencer la gamme à 4.000 ? à partir de quelle concentration le standard est-il soluble ?

    Et si oui, la qualité du test, la spécificité/sensibilité/précision du test est-elle tjs garantie ?

    Pour la MMP1, idem, est-il possible d’aller plus haut que 18 ng/mL ?

    Je vous demande cela car je souhaite analyser des surnageants de culture cellulaire, précisément de milieu de culture de traitement de tissus ex vivo. Généralement les concentrations en IL8 sont fortes, donc j’aurai besoin d’une gamme de standards qui aille le plus haut possible, pour ne pas avoir à diluer trop fortement mes surnageants et surtout que les valeurs ne soient pas toutes hors gamme.

    Read More
    Answer

    Nous ne recommandons pas de modifier les concentrations des courbes standards mais plutôt de diluer vos échantillons.

    Toutefois, si vous voulez vraiment changer les courbes standards ceci est possible. Cependant en augmentant les concentrations de la courbe standard, vous augmentez considérablement le risque de saturer l'anticorps qui peut conduire à un effet de plafonnement (une courbe non linéaire). N’oublions pas que l’anticorps de capture a aussi une limite du taux de protéine qu’il peut capturer.

    Veuillez noter que beaucoup de tests ont été réalisé pour déterminer les concentrations standards optimales pour les anticorps et donc si vous décidez de modifier les gammes de standards nous ne pourrons plus garantir que ces produits fonctionneront.

    De plus, nous ne savons pas à partir de quelle concentration les standards sont solubles.

    Pour ab100603, nous recommandons de reconstituer le standard dans 400 ul de diluant pour faire 100 ng/ml. Ceci est après dilué ˜5.555 fois pour faire une concentration de 18,000 pg/ml. Je vous conseille donc de diluer le standard de 100 ng/ml moins que 5.55 fois pour obtenir une gamme plus concentré.

    Pour ab46032, nous ne recommandons pas de reconstituer le standard dans un plus petit volume, pour obtenir 4.000 pg/mL.

    Read More

    Question

    Using this kit with urine samples. Should they be diluted with the "standard diluent-human serum"or the "standard diluent buffer"? Do you have any suggestions for the dilution factor of this kind of sample?

    Read More
    Answer

    Thank you very much for your call yesterday and for your patience while I have been in touch with the lab about ab46032.

    This kit is suitable for all body fluids. Urine samples should be diluted in standard diluent buffer (containing PBS BSA). We have no data to report on urine samples, as the titers are variable, but we suggest starting the dilution at 1:1 (50 uL of samples and 50 uL of diluent).

    I hope that this information will be useful, but please let me know if you have any further questions and I'll be happy to help.

    Read More

    Question

    Dear Tech Support Team,

    Kindly advise if the following products can be used with human ascites:

    https://www.abcam.com/High-Sensitivity-IL6-Human-ELISA-Kit-1-x-96-Well-Plate-ab46042.html

    https://www.abcam.com/High-Sensitivity-IL6-Human-ELISA-Kit-2-x-96-Well-Plates-ab46044.html

    https://www.abcam.com/IL6-Human-Elipair-Kit-Reagents-for-10-x-96-Well-Plates-ab48479.html

    https://www.abcam.com/IL6-Human-Elipair-Kit-Reagents-for-5-x-96-Well-Plates-ab48478.html

    https://www.abcam.com/IL6-Human-ELISA-Kit-ab108867.html

    https://www.abcam.com/IL6-Human-ELISA-Kit-1-x-96-Well-Plate-ab46027.html

    https://www.abcam.com/IL8-Human-ELISA-Kit-1-x-96-Well-Plate-ab46032.html

    https://www.abcam.com/IL8-Human-ELISA-Kit-ab100575.html

    https://www.abcam.com/IL8-Human-ELISA-Kit-ab108869.html

    https://www.abcam.com/IL8-Human-ELISA-Kit-2-x-96-Well-Plates-ab46049.html

    https://www.abcam.com/IL8-Human-ELISA-Kit-1-x-96-Well-Plate-ab100576.html

    Thanks in advance for your assistance and reply.
    Best Regards,

    Read More
    Answer

    Thank you for contacting us. I apologise for the delay.
    Unfortunately any of these kits have been tested with human ascites. However, they should work as they are suitable for all body fluids.
    The only problem I would suspect is the high concentration of IgG in ascites which can interfere with the ELISA. I would suggest diluting samples 1:2 or 1:5 to minimize the matrix effect.
    I hope this helps and if we can assist further, please do not hesitate to contact us.

    Read More

    Question

    Would this kit be compatible with mouse samples?

    Read More
    Answer

    Thank you for your phone call yesterday and for your questions about our ELISA kits. I have heard back from the lab regarding ab46032, and unfortunately, the kit was never assayed on murine samples. So, I would not recommend this kit to use with mouse samples, but you may be able to find a mouse IL8 kit from another vendor by searching on www.biocompare.com. Please let me know if you have any questions!

    Read More

    Question

    Can you tell me if Thimerosal is included in any of the components of this Human IL8 ELISA Kit - 1 x 96 Well Plate)? If so, which component and at what percentage?

    Read More
    Answer

    Thank you for your enquiry. There's no Thimerosal in any component of the kit. However, we use azide (0.1%) in standard diluent and Biotinylated antibody diluent, and Proclin (0.1%) in HRP diluent.

    Read More

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