人IL-12 p40 + IL-12 p70 ELISA试剂盒(ab46037)
Key features and details
- Sensitivity: 20 pg/ml
- Range: 62.5 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人IL-12 p40 + IL-12 p70 ELISA试剂盒
参阅全部 IL-12B 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 3.7% 批次间 样品 n Mean SD CV% Sample 1 18 2277pg/ml 160 7% Sample 2 18 1199pg/ml 108 9% Sample 3 18 479pg/ml 49 10.3% Sample 4 18 1488pg/ml 123 8.3% Sample 5 18 826pg/ml 106 12.8% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 20 pg/ml -
范围
62.5 pg/ml - 2000 pg/ml -
回收率
100 %
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检测时间
1h 45m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s IL-12 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-12 in Human serum, plasma, buffered solutions and supernatants.
A monoclonal antibody specific for IL-12 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-12 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-12 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-12 present in the samples.
This kit will recognize both endogenous and recombinant Human IL-12, and the Human Il-12 p40 monomer, dimer or heterodimer p70 (p40 + p35) forms of the molecule.
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 2 x 96 tests 1 x 96 tests 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 25ml 1 x 15ml 1 x 15ml 1 x 25ml 200X Wash Buffer White 2 x 10ml 1 x 10ml 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 13ml 1 x 7ml 1 x 7ml 1 x 13ml Biotinylated anti-IL12 Red 2 x 0.4ml 1 x 0.4ml 1 x 0.4ml 2 x 0.4ml Chromogen TMB Substrate Solution 1 x 24ml 1 x 11ml 1 x 11ml 1 x 24ml HRP Diluent Red 1 x 23ml 1 x 12ml 1 x 12ml 1 x 23ml IL-12 Control Silver 4 vials 2 vials 2 vials 4 vials IL-12 Microplate (12 x 8 well strips) 2 units 1 unit 1 unit 2 units IL-12 Standard (Lyophilized) Yellow 4 vials 2 vials 2 vials 4 vials Plastic Plate Covers 4 units 2 units 2 units 4 units Standard Diluent (Serum) 2 x 7ml 1 x 7ml 1 x 7ml 2 x 7ml Stop Reagent Black 2 x 11ml 1 x 11ml 1 x 11ml 2 x 11ml Streptavidin-HRP 4 x 5µl 2 x 5µl 2 x 5µl 4 x 5µl -
研究领域
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功能
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. -
疾病相关
Mendelian susceptibility to mycobacterial disease
Psoriasis 11 -
序列相似性
Belongs to the type I cytokine receptor family. Type 3 subfamily.
Contains 1 fibronectin type-III domain.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
翻译后修饰
Known to be C-mannosylated in the recombinant protein; it is not yet known for sure if the wild-type protein is also modified. -
细胞定位
Secreted. - Information by UniProt
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别名
- CLMF
- CLMF p40
- CLMF2
see all -
数据库链接
- Entrez Gene: 3593 Human
- Omim: 161561 Human
- SwissProt: P29460 Human
- Unigene: 674 Human
图片
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Typical Standard Curve
Representative Standard Curve using ab46037
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab46037 被引用在 1 文献中.
- Gu Y et al. Enhancing Fatty Acid Catabolism of Macrophages Within Aberrant Breast Cancer Tumor Microenvironment Can Re-establish Antitumor Function. Front Cell Dev Biol 9:665869 (2021). PubMed: 33937269