人IL-1 beta酶联免疫斑点法（ELISPOT）Set (ab46578)
Key features and details
- Assay type: Sandwich (qualitative)
- Sample type: Suspension cells
- Reacts with: Human
概述
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产品名称
人IL-1 beta酶联免疫斑点法（ELISPOT）Set
参阅全部 IL-1 beta 试剂盒 -
样品类型
Suspension cells -
检测类型
Sandwich (qualitative) -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
The Human IL-1 beta ELISPOT Set assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.
This ELISPOT assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.
Principal:
After cell stimulation, locally produced cytokines are captured by a specific monoclonal antibody. After cell lysis, trapped cytokine molecules are revealed by a secondary biotinylated detection antibody, which is in turn recognised by streptavidin conjugated to alkaline phosphatase. PVDF-bottomed-well plates are then incubated with BCIP/NBT substrate. Colored "purple" spots indicate cytokine production by individual cells.
Recognizes natural (pro and mature) human IL-1 beta.
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经测试应用
适用于: ELISpotmore details -
平台
Reagents
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 5 x 96 tests 10 x 96 tests 15 x 96 tests 20 x 96 tests Bovine Serum Albumin 1 x 1g 2 x 1g 3 x 1g 4 x 1g Human IL-1β Capture antibody 1 x 500µl 2 x 500µl 3 x 500µl 4 x 500µl IL-1ß Biotinylated Detection antibody 1 vial 2 vials 3 vials 4 vials Ready-to-use BCIP/NBT substrate buffer 1 x 50ml 2 x 50ml 3 x 50ml 4 x 50ml Streptavidin - Alkaline Phosphatase conjugated 1 x 50µl 2 x 50µl 3 x 50µl 4 x 50µl -
研究领域
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功能
Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. -
组织特异性
Expressed in activated monocytes/macrophages (at protein level). -
序列相似性
Belongs to the IL-1 family. -
翻译后修饰
Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated. -
细胞定位
Cytoplasm, cytosol. Lysosome. Secreted, exosome. Cytoplasmic vesicle, autophagosome. Secreted. The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive. - Information by UniProt
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别名
- Catabolin
- H1
- IFN beta inducing factor
see all -
数据库链接
- Entrez Gene: 3553 Human
- Omim: 147720 Human
- SwissProt: P01584 Human
- Unigene: 126256 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab46578于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISpot |
Use at an assay dependent dilution.
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| 说明 |
|---|
|
ELISpot
Use at an assay dependent dilution. |
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab46578 被引用在 1 文献中.
- Dissanayake K et al. Potential applicability of cytokines as biomarkers of disease activity in rheumatoid arthritis: Enzyme-linked immunosorbent spot assay-based evaluation of TNF-a, IL-1ß, IL-10 and IL-17A. PLoS One 16:e0246111 (2021). PubMed: 33497394