人IL-1 beta ELISA试剂盒(ab46052)
Key features and details
- Sensitivity: 6.5 pg/ml
- Range: 15.6 pg/ml - 500 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
-
产品名称
人IL-1 beta ELISA试剂盒
参阅全部 IL-1 beta 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% 6 4.5% 批次间 样品 n Mean SD CV% 6 8.7% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
6.5 pg/ml -
范围
15.6 pg/ml - 500 pg/ml -
回收率
102.2 %
特定样本回收率 样品类型 平均% 范围 Serum 102.2 15.6pg/ml - 500pg/ml -
检测时间
3h 45m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Human IL-1 beta ab46052 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-1 beta in Human serum, plasma, buffered solutions or cell culture medium.
A monoclonal antibody specific for IL-1 beta has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-1 beta concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-1 beta are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-1 beta present in the samples.
This kit will recognize both endogenous and recombinant Human IL-1 beta.
-
平台
Microplate
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 1 x 96 tests 2 x 96 tests 2 x 96 tests 1 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 1 x 25ml 1 x 15ml 200X Wash Buffer White 1 x 10ml 2 x 10ml 2 x 10ml 1 x 10ml Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml 1 x 13ml 1 x 7.5ml Biotinylated anti-IL-1 beta Red 1 x 400µl 2 x 400µl 2 x 400µl 1 x 400µl Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml 1 x 24ml 1 x 11ml Control Silver 2 vials 4 vials 4 vials 2 vials HRP Diluent Red 1 x 12ml 1 x 23ml 1 x 23ml 1 x 12ml IL-1 beta Microplate (12 x 8 well strips) 1 unit 2 units 2 units 1 unit IL1 beta standard Yellow 2 vials 4 vials 4 vials 2 vials Standard Diluent (Serum) 1 x 7ml 2 x 7ml 2 x 7ml 1 x 7ml Stop Reagent Black 1 x 11ml 2 x 11ml 2 x 11ml 1 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl 4 x 5µl 2 x 5µl -
研究领域
-
功能
Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. -
组织特异性
Expressed in activated monocytes/macrophages (at protein level). -
序列相似性
Belongs to the IL-1 family. -
翻译后修饰
Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated. -
细胞定位
Cytoplasm, cytosol. Lysosome. Secreted, exosome. Cytoplasmic vesicle, autophagosome. Secreted. The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive. - Information by UniProt
-
别名
- Catabolin
- H1
- IFN beta inducing factor
see all -
数据库链接
- Entrez Gene: 3553 Human
- Omim: 147720 Human
- SwissProt: P01584 Human
- Unigene: 126256 Human
图片
-
ELISA: IL-1 beta Human ELISA Kit (ab46052)
Standard curve of human IL-1β in standard diluent with background signal subtracted (duplicates; +/- SD).
-
ELISA: IL-1 beta Human ELISA Kit (ab46052)IL-1β detected in supernatants from control THP-1 cells (-) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) and 1 ug x mL-1 LPS (Sigma) for the last 6 hours (P+L).
-
Typical Standard CurveRepresentative Standard Curve using ab46052
数据表及文件
-
SDS download
-
Datasheet download
文献 (66)
ab46052 被引用在 66 文献中.
- Hsueh YH et al. Therapeutic effect of induced pluripotent stem cell -derived extracellular vesicles in an in vitro and in vivo osteoarthritis model. J Orthop Translat 38:141-155 (2023). PubMed: 36381245
- Ji J et al. lncRNA OIP5-AS1 knockdown or miR-223 overexpression can alleviate LPS-induced ALI/ARDS by interfering with miR-223/NLRP3-mediated pyroptosis. J Gene Med 24:e3385 (2022). PubMed: 34346534
- Ye T et al. LncRNA CCAT1 is overexpressed in tuberculosis patients and predicts their survival. Immun Inflamm Dis 10:218-224 (2022). PubMed: 34847295
- Cansby E et al. Silencing of STE20-type kinase STK25 in human aortic endothelial and smooth muscle cells is atheroprotective. Commun Biol 5:379 (2022). PubMed: 35440683
- Chen W et al. The Molecular Mechanism of Long Non-Coding RNA MALAT1-Mediated Regulation of Chondrocyte Pyroptosis in Ankylosing Spondylitis. Mol Cells 45:365-375 (2022). PubMed: 35680372