人IL-1 alpha ELISA试剂盒(ab46028)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 31.2 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人IL-1 alpha ELISA试剂盒
参阅全部 IL-1 alpha 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Sample A 22 459.8pg/ml 13 2.8% Sample B 21 72.6pg/ml 3.9 4.3% 批次间 样品 n Mean SD CV% Sample A 8 259.9pg/ml 15 5.8% Sample B 8 58.6pg/ml 4.3 7.3% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 10 pg/ml -
范围
31.2 pg/ml - 1000 pg/ml -
回收率
95.3 %
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检测时间
3h 45m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s IL-1 alpha Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-1 alpha in cell culture supernatants, buffered solutions, serum, plasma and other body fluids. This assay will recognize both natural and recombinant Human IL-1a.
A monoclonal antibody specific for IL-1 alpha has been coated to the wells of the microtiter strip plate provided. Samples, including standards of known IL-1 alpha concentrations and unknown are pipetted into these wells. During incubation, binding of IL-1 alpha to the capture antibodies is completed and then any excess unbound analyte is removed by washing. A biotinylated monoclonal antibody specific for IL-1 alpha is then incubated. After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-1 alpha present in the sample.
This kit will recognize both endogenous and recombinant Human IL-1 alpha.
Get higher sensitivity in only 90 minutes with Human IL-1 alpha ELISA Kit (ab178008) from our SimpleStep ELISA® range.
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 2 x 96 tests 1 x 96 tests 10X Standard Diluent Buffer Black 1 x 25ml 1 x 15ml 200X Wash Buffer White 2 x 10ml 1 x 10ml Biotinylated Antibody Diluent Red 1 x 13ml 1 x 7ml Biotinylated anti-IL-1 alpha Red 2 x 0.4ml 1 x 0.4ml Chromogen TMB Substrate Solution 1 x 24ml 1 x 11ml HRP Diluent Red 1 x 23ml 1 x 12ml IL-1 alpha Control Silver 4 vials 2 vials IL-1 alpha Microplate (12 x 8 well strips) 2 units 1 unit IL-1 alpha Standard (Lyophilized) Yellow 4 vials 2 vials Standard Diluent (Serum) 2 x 7ml 1 x 7ml Stop Reagent Black 2 x 11ml 1 x 11ml Streptavidin-HRP 4 x 5µl 2 x 5µl -
研究领域
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相关性
IL-1 alpha is a member of the interleukin 1 cytokine family. It is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. -
细胞定位
Secreted -
别名
- Hematopoietin 1
- IL 1
- IL 1 alpha
see all -
数据库链接
- Entrez Gene: 3552 Human
- Omim: 147760 Human
- SwissProt: P01583 Human
相关产品
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SimpleStep ELISA kits
图片
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Typical Standard Curve
Representative Standard Curve using ab46028
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab46028 被引用在 1 文献中.
- Zhong Y et al. Blueberry Improves the Therapeutic Effect of Etanercept on Patients with Juvenile Idiopathic Arthritis: Phase III Study. Tohoku J Exp Med 237:183-91 (2015). PubMed: 26477692