人IL-1 alpha ELISA试剂盒(ab100560)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 0.82 pg/ml - 600 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人IL-1 alpha ELISA试剂盒
参阅全部 IL-1 alpha 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 2 pg/ml -
范围
0.82 pg/ml - 600 pg/ml -
回收率
95 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 102.25 89% - 108% Serum 91.89 80% - 101% Plasma 92.46 82% - 102% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s IL-1 alpha Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-1 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human IL-1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Human IL-1a ELISA Kit (ab178008) from our SimpleStep ELISA® range.
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说明
Optimization may be required with urine samples.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 500X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Human IL-1 alpha 2 vials IL-1 alpha Microplate (12 x 8 wells) 1 unit Recombinant Human IL-1 alpha Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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相关性
IL-1 alpha is a member of the interleukin 1 cytokine family. It is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. -
细胞定位
Secreted -
别名
- Hematopoietin 1
- IL 1
- IL 1 alpha
see all -
数据库链接
- Entrez Gene: 3552 Human
- Omim: 147760 Human
- SwissProt: P01583 Human
相关产品
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SimpleStep ELISA kits
图片
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Typical standard curve
Representative standard curve using ab100560
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Typical standard curveRepresentative standard curve using ab100560
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab100560 被引用在 1 文献中.
- Yang H et al. Ovarian IL-1a and IL-1ß levels are associated with primary ovarian insufficiency. Int J Clin Exp Pathol 11:4711-4717 (2018). PubMed: 31949872