Key features and details
- Sensitivity: 2 pg/ml
- Range: 0.82 pg/ml - 600 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
产品名称人IL-1 alpha ELISA试剂盒
参阅全部 IL-1 alpha 试剂盒
样品类型Cell culture supernatant, Serum, Plasma
灵敏度< 2 pg/ml
范围0.82 pg/ml - 600 pg/ml
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 102.25 89% - 108% Serum 91.89 80% - 101% Plasma 92.46 82% - 102%
实验步骤Multiple steps standard assay
Abcam’s IL-1 alpha Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-1 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human IL-1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Human IL-1a ELISA Kit (ab178008) from our SimpleStep ELISA® range.
Optimization may be required with urine samples.
存放说明Store at -20°C. Please refer to protocols.
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 500X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Human IL-1 alpha 2 vials IL-1 alpha Microplate (12 x 8 wells) 1 unit Recombinant Human IL-1 alpha Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
相关性IL-1 alpha is a member of the interleukin 1 cytokine family. It is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease.
- Hematopoietin 1
- IL 1
- IL 1 alpha
SimpleStep ELISA kits
ab100560 被引用在 1 文献中.
- Yang H et al. Ovarian IL-1a and IL-1ß levels are associated with primary ovarian insufficiency. Int J Clin Exp Pathol 11:4711-4717 (2018). PubMed: 31949872