人ICAM3 ELISA试剂盒(CD50) (ab46024)
Key features and details
- Sensitivity: 0.3 ng/ml
- Range: 0.62 ng/ml - 20 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人ICAM3 ELISA试剂盒(CD50) -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Sample A 8 19.75ng/ml 0.172 0.86% Sample B 8 2.83ng/ml 0.099 3.49% 批次间 样品 n Mean SD CV% Sample A 36 19.51ng/ml 0.391 1.99% Sample B 36 2.71ng/ml 0.176 6.27% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 0.3 ng/ml -
范围
0.62 ng/ml - 20 ng/ml -
检测时间
1h 40m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s ICAM3 (CD50) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of ICAM3 in serum, plasma, buffered solutions and cell culture medium.
A monoclonal antibody specific for ICAM3 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known ICAM3 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for ICAM3 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of ICAM3 present in the samples.
This kit will recognize both endogenous and recombinant Human ICAM3.
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml Biotinylated anti-ICAM-3 (CD50) Red 1 x 0.4ml 2 x 0.4ml Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml HRP Diluent Red 1 x 12ml 1 x 23ml ICAM3 Control Silver 2 vials 4 vials ICAM3 Microplate (12 x 8 well strips) 1 unit 2 units ICAM3 Standard Yellow 2 vials 4 vials Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl -
研究领域
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相关性
ICAM3 is a member of the intercellular adhesion molecule family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. ICAM3 is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. -
细胞定位
Cell Membrane; single-pass type I membrane protein -
别名
- CD50
- CDW50
- ICAM 3
see all -
数据库链接
- Entrez Gene: 3385 Human
- SwissProt: P32942 Human
图片
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Typical Standard Curve
Representative Standard Curve using ab46024
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab46024 尚未被引用在任何文献中。