人ICAM2 ELISA试剂盒(CD102) (ab46080)
Key features and details
- Sensitivity: 0.2 U/ml
- Range: 0.75 U/ml - 24 U/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人ICAM2 ELISA试剂盒(CD102)
参阅全部 ICAM2 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% A 8 23.62U/ml 0.277 1.17% B 8 2.96U/ml 0.86 2.9% 批次间 样品 n Mean SD CV% A 33 23.26U/ml 0.668 2.87% B 33 3.12 0.218 6.98% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
= 0.2 U/ml -
范围
0.75 U/ml - 24 U/ml -
检测时间
1h 40m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Human ICAM2 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of soluble Inter Cellular Adhesion Molecule -2 (ICAM2) in Human serum, plasma, buffered solutions or cell culture medium.
A monoclonal antibody specific for ICAM2 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known ICAM2 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for ICAM2 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of ICAM2 present in the samples.
This kit will recognize both endogenous and recombinant Human ICAM2.
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml Biotinylated anti-ICAM2 Red 1 x 400µl 2 x 400µl Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml Control Silver 2 vials 4 vials HRP Diluent Red 1 x 12ml 1 x 23ml ICAM2 Microplate (12 x 8 well strips) 1 unit 2 units ICAM2 Standard (Lyophilized) Yellow 2 vials 4 vials Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl -
研究领域
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相关性
ICAM2 is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin like C2 type domains, and bind to the leukocyte adhesion LFA1 protein. This protein may play a role in lymphocyte recirculation by blocking LFA1 dependent cell adhesion. It mediates adhesive interactions important for antigen specific immune response, NK cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. -
细胞定位
Type 1 membrane protein -
别名
- CD102
- ICAM 2
- Intercellular adhesion molecule 2
- Ly60
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数据库链接
- Entrez Gene: 3384 Human
- Omim: 146630 Human
- SwissProt: P13598 Human
图片
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Typical Standard Curve
Representative Standard Curve using ab46080
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab46080 尚未被引用在任何文献中。