人ICAM1 ELISA试剂盒(CD54) (ab100640)
Key features and details
- Sensitivity: 150 pg/ml
- Range: 150 pg/ml - 20000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人ICAM1 ELISA试剂盒(CD54)
参阅全部 ICAM1 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 150 pg/ml -
范围
150 pg/ml - 20000 pg/ml -
回收率
97 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 97.37 89% - 107% Serum 97.48 88% - 106% Plasma 98.67 90% - 107% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s ICAM1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human soluble ICAM1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human ICAM1 coated on a 96-well plate. Standards and samples are pipetted into the wells and ICAM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human ICAM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ICAM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Human ICAM1 ELISA Kit (CD54) (ab174445) from our SimpleStep ELISA® range.
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说明
Optimization may be required with urine samples.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 340X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human ICAM1 2 vials ICAM1 Microplate (12 x 8 wells) 1 unit Recombinant Human ICAM1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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相关性
ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria. -
细胞定位
Membrane -
别名
- BB2
- CD 54
- CD54
see all -
数据库链接
- Entrez Gene: 3383 Human
- Omim: 147840 Human
- SwissProt: P05362 Human
相关产品
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SimpleStep ELISA kits
图片
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Typical Standard Curve
Representative standard curve using ab100640
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Typical Standard CurveRepresentative standard curve using ab100640
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab100640 被引用在 7 文献中.
- Resano A et al. Elovanoids Counteract Inflammatory Signaling, Autophagy, Endoplasmic Reticulum Stress, and Senescence Gene Programming in Human Nasal Epithelial Cells Exposed to Allergens. Pharmaceutics 14:N/A (2022). PubMed: 35057008
- Ounjaijean S et al. Cardiovascular risks in Asian HIV-infected patients receiving boosted-protease inhibitor-based antiretroviral treatment. J Infect Dev Ctries 15:289-296 (2021). PubMed: 33690213
- Hosseinkhani B et al. (Sub)populations of extracellular vesicles released by TNF-a -triggered human endothelial cells promote vascular inflammation and monocyte migration. J Extracell Vesicles 9:1801153 (2020). PubMed: 32944190
- Strewe C et al. PlanHab Study: Consequences of combined normobaric hypoxia and bed rest on adenosine kinetics. Sci Rep 8:1762 (2018). PubMed: 29379127
- Wang N et al. Herbal Medicine Cordyceps sinensis Improves Health-Related Quality of Life in Moderate-to-Severe Asthma. Evid Based Complement Alternat Med 2016:6134593 (2016). PubMed: 28050193
- Mascarenhas-Melo F et al. Diabetes abrogates sex differences and aggravates cardiometabolic risk in postmenopausal women. Cardiovasc Diabetol 12:61 (2013). PubMed: 23570342
- Mascarenhas-Melo F et al. Emergent Biomarkers of Residual Cardiovascular Risk in Patients with Low HDL-c and/or High Triglycerides and Average LDL-c Concentrations: Focus on HDL Subpopulations, Oxidized LDL, Adiponectin, and Uric Acid. ScientificWorldJournal 2013:387849 (2013). PubMed: 24319364