人hCG ELISA试剂盒(ab100533)
Key features and details
- Sensitivity: 50 pg/ml
- Range: 54.87 pg/ml - 40000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人hCG ELISA试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 50 pg/ml -
范围
54.87 pg/ml - 40000 pg/ml -
回收率
> 90 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 130 120% - 135% Serum 113.7 103% - 124% Plasma 90.57 83% - 103% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s hCG Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human hCG in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human hCG coated on a 96-well plate. Standards and samples are pipetted into the wells and hCG present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human hCG antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of hCG bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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说明
Optimization may be required with urine samples
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 700X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Human hCG 2 vials hCG Microplate (12 x 8 wells) 1 unit Human hCG Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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相关性
Human chorionic gonadotropin (hCG) is a glycoprotein hormone produced by trophoblastic cells of the placenta beginning 10 to 12 days after conception. Maintenance of the fetus in the first trimester of pregnancy requires the production of hCG, which binds to the corpus luteum of the ovary which is stimulated to produce progesterone which in turn maintains the secretory endometrium. hCG is present only in trace amounts in non pregnant urine and sera. It rises sharply during pregnancy. HCG is composed of two non identical, non covalently linked polypeptide chains designated as the a and b subunits. The a subunit of HCG is nearly identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH) and luteinizing hormone (LH). A germ cell tumor which is positive for cytokeratin, placental alkaline phosphatase (PLAP) and HCG but negative for EMA and AFP is probably a choriocarcinoma. -
别名
- Anterior pituitary glycoprotein hormones common subunit alpha
- CG ALPHA
- CG beta
see all -
数据库链接
- SwissProt: P01233 Human
- SwissProt: P01215 Human
图片
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Typical standard curve
Representative standard curve using ab100533
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Typical standard curveRepresentative standard curve using ab100533
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab100533 被引用在 14 文献中.
- Cui K et al. Establishment of Trophoblast-Like Tissue Model from Human Pluripotent Stem Cells in Three-Dimensional Culture System. Adv Sci (Weinh) 9:e2100031 (2022). PubMed: 34813178
- Io S et al. Capturing human trophoblast development with naive pluripotent stem cells in vitro. Cell Stem Cell 28:1023-1039.e13 (2021). PubMed: 33831365
- Sato M et al. Three-dimensional human placenta-like bud synthesized from induced pluripotent stem cells. Sci Rep 11:14167 (2021). PubMed: 34239021
- Skjærvø Ø et al. All-in-one paper-based sampling chip for targeted protein analysis. Anal Chim Acta 1089:56-65 (2019). PubMed: 31627819
- Tseng AM et al. Maternal circulating miRNAs that predict infant FASD outcomes influence placental maturation. Life Sci Alliance 2:N/A (2019). PubMed: 30833415