Key features and details
- Sensitivity: 20 pg/ml
- Range: 31.25 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
产品名称人Granzyme B ELISA试剂盒(CTLA-1)
批次内 样品 n Mean SD CV% A 15 356pg/ml 18 5.06% B 15 73pg/ml 5 7.38% 批次间 样品 n Mean SD CV% A 8 536pg/ml 30 5.59% B 8 138pg/ml 9 6.76%
样品类型Cell culture supernatant, Serum, Plasma
灵敏度< 20 pg/ml
范围31.25 pg/ml - 1000 pg/ml
特定样本回收率 样品类型 平均% 范围 Serum 73 31.25pg/ml - 1000pg/ml
实验步骤Multiple steps standard assay
Abcam’s Granzyme B (CTLA-1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Granzyme B in Human serum, plasma, buffered solutions and cell culture medium.
A monoclonal antibody specific Granzyme B has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Granzyme B concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for Granzyme B are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of Granzyme B present in the samples.
This kit will recognize both endogenous and recombinant Human Granzyme B.
存放说明Store at +4°C. Please refer to protocols.
组件 标识符 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml Biotinylated anti-Granzyme B Red 1 x 400µl 2 x 400µl Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml Granzyme B Microplate (12 x 8 well strips) 1 unit 2 units Granzyme B Standard (Lyophilized) Yellow 2 vials 4 vials HRP Diluent Red 1 x 12ml 1 x 23ml Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl
相关性The lytic enzymes of cytotoxic lymphocytes seem to be contained within lysosome-like granules found in the cytoplasm. These granules contain perforin, a membrane pore-forming protein, and a family of serine proteases (granzymes). Granzyme B, a 32 kDa protein, is a serine Aspase associated with the granules of Natural Killer (NK) cells. Granzyme B seems to be able to elicit cell death, perhaps by activating an endogenous pathway of programmed cell death.
- CTLA 1
- Fragmentin 2
ab46142 被引用在 8 文献中.
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