人GM-CSF Receptor alpha ELISA试剂盒(CD116) (ab46086)
Key features and details
- Sensitivity: 16 pg/ml
- Range: 31.25 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人GM-CSF Receptor alpha ELISA试剂盒(CD116)
参阅全部 GM-CSF Receptor alpha 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% A 31 700.2pg/ml 20.4 2.9% B 32 378pg/ml 12.8 3.4% 批次间 样品 n Mean SD CV% A 6 555pg/ml 22.2 4% B 6 120.7pg/ml 2.2 1.8% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 16 pg/ml -
范围
31.25 pg/ml - 1000 pg/ml -
检测时间
3h 45m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s GM-CSF Receptor alpha (CD116) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of GM-CSF R alpha in serum, plasma, and cell culture supernatants.
A monoclonal antibody specific for GM-CSF R alpha has been coated onto the wells of the microtiter strips provided. Samples, including standards of known GM-CSF R alpha concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for GM-CSF R alpha are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of GM-CSF R alpha present in the samples.
This kit will recognize both endogenous and recombinant Human GM-CSF R alpha.
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 2 x 96 tests 1 x 96 tests 1 x 96 tests 10X Standard Diluent Buffer Black 1 x 25ml 1 x 15ml 1 x 15ml 200X Wash Buffer White 2 x 10ml 1 x 10ml 1 x 10ml Biotinylated Antibody Diluent Red 1 x 13ml 1 x 7ml 1 x 7ml Biotinylated anti-GM-CSF R alpha Red 2 x 400µl 1 x 400µl 1 x 400µl Chromogen TMB Substrate Solution 1 x 24ml 1 x 11ml 1 x 11ml Control Silver 4 vials 2 vials 2 vials GM-CSF Receptor alpha Microplate (12 x 8 well strips) 2 units 1 unit 1 unit GM-CSF Receptor alpha Standard (Lyophilized) Yellow 4 vials 2 vials 2 vials HRP Diluent Red 1 x 23ml 1 x 12ml 1 x 12ml Stop Reagent Black 2 x 11ml 1 x 11ml 1 x 11ml Streptavidin-HRP 4 x 5µl 2 x 5µl 2 x 5µl -
研究领域
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相关性
GM-CSF Receptor alpha is the alpha subunit of the heterodimeric receptor for colony stimulating factor 2, a cytokine which controls the production, differentiation, and function of granulocytes and macrophages. The encoded protein is a member of the cytokine family of receptors. This gene is found in the pseudoautosomal region of the X and Y chromosomes. Multiple transcript variants encoding different isoforms have been found for this gene, with some of the isoforms being membrane-bound and others being soluble. -
细胞定位
Cell membrane; single-pass type I membrane protein. Isoform 3, isoform 4, isoform 6: Secreted protein -
别名
- CD 116
- CD116
- CD116 antigen
see all -
数据库链接
- Entrez Gene: 1438 Human
- SwissProt: P15509 Human
图片
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Typical Standard Curve
Representative Standard Curve using ab46086
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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