人Factor IX/PTC ELISA试剂盒(ab300307)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 15.884 pg/ml
- Range: 97.656 pg/ml - 6250 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Factor IX/PTC ELISA试剂盒
参阅全部 Factor IX/PTC 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Serum 8 7.4% 批次间 样品 n Mean SD CV% Serum 3 10.1% -
样品类型
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
15.884 pg/ml -
范围
97.656 pg/ml - 6250 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 105 92% - 113% Hep Plasma 112 103% - 118% EDTA Plasma 106 95% - 115% Cit plasma 116 111% - 119% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Human Factor IX/PTC SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Factor IX/PTC protein in human serum and plasma. Quantitate Human Factor IX/PTC with 15.884 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 10 x 96 tests 96 tests 1 x 384 tests 10X Human Factor IX/PTC Capture Antibody 1 x 6000µl 1 x 600µl 1 x 600µl 10X Human Factor IX/PTC Detector Antibody 1 x 6000µl 1 x 600µl 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 200ml 1 x 20ml 1 x 20ml 384 well CaptSure™ microplates 0 x 0 unit 0 x 0 unit 1 unit Antibody Diluent 4BI 10 x 6ml 1 x 6ml 1 x 6ml Human Factor IX/PTC Lyophilized Recombinant Protein 2 x 10 vials 2 vials 2 vials Plate Seals 1 x 10 units 1 unit 1 unit Sample Diluent NS (ab193972) 2 x 250ml 1 x 50ml 2 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 x 10 units 1 unit 0 x 0 unit Stop Solution 1 x 120ml 1 x 12ml 2 x 12ml TMB Development Solution 1 x 120ml 1 x 12ml 2 x 12ml -
研究领域
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功能
Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa. -
组织特异性
Synthesized primarily in the liver and secreted in plasma. -
疾病相关
Defects in F9 are the cause of recessive X-linked hemophilia B (HEMB) [MIM:306900]; also known as Christmas disease.
Note=Mutations in position 43 (Oxford-3, San Dimas) and 46 (Cambridge) prevents cleavage of the propeptide, mutation in position 93 (Alabama) probably fails to bind to cell membranes, mutation in position 191 (Chapel-Hill) or in position 226 (Nagoya OR Hilo) prevent cleavage of the activation peptide.
Defects in F9 are the cause of thrombophilia due to factor IX defect (THR-FIX) [MIM:300807]. A hemostatic disorder characterized by a tendency to thrombosis. -
序列相似性
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain. -
结构域
Calcium binds to the gamma-carboxyglutamic acid (Gla) residues and, with stronger affinity, to another site, beyond the Gla domain. -
翻译后修饰
Activated by factor XIa, which excises the activation peptide.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
细胞定位
Secreted. - Information by UniProt
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别名
- Christmas Disease
- Christmas factor
- Coagulant factor IX
see all -
数据库链接
- Entrez Gene: 2158 Human
- Omim: 300746 Human
- SwissProt: P00740 Human
- Unigene: 522798 Human
图片
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Interpolated concentration of native Factor IX/PTC was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1:400 serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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Example of human Factor IX/PTC standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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Example of human Factor IX/PTC standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentration of native Factor IX/PTC was measured in duplicate at different sample concentrations. Undiluted samples are as follows: serum 0.15%, plasma (citrate) 0.075%, plasma (EDTA) 0.075%, and plasma (heparin) 0.15%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean Factor IX/PTC concentration was determined to be 4,867.18 ng/mL with a range of 1,062.16 – 8,981.25 ng/mL.
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Standard curve comparison between the original Human Factor IX/PTC SimpleStep ELISA (ab188393) and current Human Factor IX/PTC SimpleStep ELISA (ab300307).
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Serum and plasma comparison between the original Human Factor IX/PTC SimpleStep ELISA (ab188393) and current Human Factor IX/PTC SimpleStep ELISA (ab300307).
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
数据表及文件
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SDS download
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Datasheet download
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