人DR5 ELISA试剂盒(CD262) (ab46013)
Key features and details
- Sensitivity: 6 pg/ml
- Range: 31.25 pg/ml - 1000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人DR5 ELISA试剂盒(CD262)
参阅全部 DR5 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Sample A 8 483pg/ml 7 1.39% Sample B 8 117pg/ml 4 3.23% 批次间 样品 n Mean SD CV% Sample A 9 679pg/ml 45 6.58% Sample B 9 186pg/ml 14 7.44% -
样品类型
Cell culture supernatant, Serum, Cell culture extracts -
检测类型
Sandwich (quantitative) -
灵敏度
< 6 pg/ml -
范围
31.25 pg/ml - 1000 pg/ml -
检测时间
2h 30m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s DR5 (CD262) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of DR5 in Human serum, plasma, buffered solutions and cell culture media.
A monoclonal antibody specific for DR5 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known DR5 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for DR5 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of DR5 present in the samples.
This kit will recognize both endogenous and recombinant Human DR5.
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 2 x 96 tests 1 x 96 tests 10X Standard Diluent Buffer Black 1 x 25ml 1 x 15ml 200X Wash Buffer White 2 x 10ml 1 x 10ml Biotinylated Antibody Diluent Red 1 x 13ml 1 x 7ml Biotinylated anti-DR5 (CD262) Red 2 x 0.4ml 1 x 0.4ml Chromogen TMB Substrate Solution 1 x 24ml 1 x 11ml DR5 Microplate (12 x 8 well strips) 2 units 1 unit DR5 Standard (Lyophilized) Yellow 4 vials 2 vials HRP Diluent Red 1 x 23ml 1 x 12ml Stop Reagent Black 2 x 11ml 1 x 11ml Streptavidin-HRP 4 x 5µl 2 x 5µl -
研究领域
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功能
Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis. -
组织特异性
Widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines such as HeLaS3, K-562, HL-60, SW480, A-549 and G-361; highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throughout the intestinal tract; not detectable in brain. -
疾病相关
Squamous cell carcinoma of the head and neck -
序列相似性
Contains 1 death domain.
Contains 3 TNFR-Cys repeats. -
细胞定位
Membrane. - Information by UniProt
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别名
- Fas like protein
- Apoptosis inducing protein TRICK2A/2B
- Apoptosis inducing receptor TRAIL R2
see all -
数据库链接
- Entrez Gene: 8795 Human
- Omim: 603612 Human
- SwissProt: O14763 Human
- Unigene: 521456 Human
- Unigene: 661668 Human
图片
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Typical Standard Curve
Representative Standard Curve using ab46013
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab46013 被引用在 1 文献中.
- Meng Q et al. Novel markers for circulating tumor stem cells in colorectal carcinoma. Am J Transl Res 8:4233-4241 (2016). PubMed: 27830007