人DcR1 ELISA试剂盒(CD263) (ab46016)
Key features and details
- Sensitivity: 147 pg/ml
- Range: 312.5 pg/ml - 10000 pg/ml
- Sample type: Cell culture extracts, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人DcR1 ELISA试剂盒(CD263) -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Sample A 8 7171pg/ml 192 2.7% Sample B 8 3584pg/ml 244 6.8% 批次间 样品 n Mean SD CV% Sample A 9 7311pg/ml 515 7% Sample B 9 2654pg/ml 356 9.7% -
样品类型
Serum, Cell culture extracts -
检测类型
Sandwich (quantitative) -
灵敏度
< 147 pg/ml -
范围
312.5 pg/ml - 10000 pg/ml -
检测时间
3h 40m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s DcR1 (CD263) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of DcR1 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
A monoclonal antibody specific for DcR1 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known DcR1 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for DcR1 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of DcR1 present in the samples.
This kit will recognize both endogenous and recombinant Human DcR1.
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml Biotinylated anti-DcR1 Red 1 x 400µl 2 x 400µl Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml DcR1 Microplate (12 x 8 well strips) 1 unit 2 units DcR1 Standard (Lyophilized) Yellow 2 vials 4 vials HRP Diluent Red 1 x 12ml 1 x 23ml Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl -
研究领域
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相关性
DcR1 is a member of the TNF-receptor superfamily. It contains an extracellular TRAIL-binding domain and a transmembrane domain, but no cytoplasmic death domain. It is not capable of inducing apoptosis, and is thought to function as an antagonistic receptor that protects cells from TRAIL-induced apoptosis. This gene was found to be a p53-regulated DNA damage-inducible gene. The expression of this gene was detected in many normal tissues but not in most cancer cell lines, which may explain the specific sensitivity of cancer cells to the apoptosis-inducing activity of TRAIL. -
细胞定位
Cell membrane; lipid-anchor; GPI-anchor -
别名
- CD263
- DCR1
- Decoy receptor 1
see all -
数据库链接
- Entrez Gene: 8794 Human
- SwissProt: O14798 Human
图片
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Typical Standard Curve
Representative Standard Curve using ab46016
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab46016 被引用在 1 文献中.
- Anand A et al. Does DcR1 (TNF-related apoptosis-inducing-ligand Receptor 3) have any role in human AMD pathogenesis? Sci Rep 4:4114 (2014). PubMed: 24534820