Key features and details
- Sensitivity: 2 pg/ml
- Range: 2 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
产品名称人CXCL1 ELISA试剂盒(GRO alpha)
参阅全部 CXCL1/GRO alpha 试剂盒
样品类型Cell culture supernatant, Serum, Plasma
范围2 pg/ml - 1000 pg/ml
> 99 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 112.2 101% - 122% Serum 130.4 119% - 145% Plasma 113.5 85% - 140%
实验步骤Multiple steps standard assay
Abcam’s CXCL1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit (GRO alpha) is an in vitro enzyme designed for the quantitative measurement of Human GRO alpha in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human CXCL1 (GRO alpha) coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CXCL1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Human CXCL1 ELISA Kit (GRO alpha) (ab190805) from our SimpleStep ELISA® range.
Optimization may be required with urine samples
存放说明Store at -20°C. Please refer to protocols.
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Biotinylated anti-Human GRO-alpha 2 vials GRO-alpha Microplate (12 x 8 wells) 1 unit Recombinant Human GRO-alpha Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
功能Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.
序列相似性Belongs to the intercrine alpha (chemokine CxC) family.
翻译后修饰N-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
- Information by UniProt
- C-X-C motif chemokine 1
- chemokine (C-X-C motif) ligand 1
- Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
SimpleStep ELISA kits
ab100530 被引用在 2 文献中.
- Winkler MJ et al. Functional investigation of the coronary artery disease gene SVEP1. Basic Res Cardiol 115:67 (2020). PubMed: 33185739
- Li L et al. Increased ROS production in non-polarized mammary epithelial cells induces monocyte infiltration in 3D culture. J Cell Sci 130:190-202 (2017). PubMed: 27656113