人CD62E ELISA试剂盒(without plates) (ab47348)
概述
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产品名称
人CD62E ELISA试剂盒(without plates) -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 0.5 ng/ml -
范围
1 ng/ml - 32 ng/ml -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s CD26E Human in vitro ELISA Set is designed for the quantitative measurement of CD26E in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
The CD62E ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
This ELISA set is intended to measure human CD62E in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
Recognizes both natural and recombinant human CD62E.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Reagents
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 10 x 96 tests 5 x 96 tests CD62E Capture Antibody 2 x 0.5ml 1 x 0.5ml CD62E standard Yellow 10 vials 5 vials Chromogen TMB Substrate Solution 4 vials 2 vials Detection biotinylated anti-CD62E antibody 2 x 0.55ml 1 x 0.55ml Streptavidin-HRP 2 vials 1 vial -
研究领域
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相关性
CD62E is expressed on cytokine induced endothelial cells and mediates their binding to leukocytes. The ligand recognized by CD62E is sialyl-lewis X (alpha(1->3)fucosylated derivatives of polylactosamine that are found at the nonreducing termini of glycolipids). -
细胞定位
Membrane; single-pass type I membrane protein -
别名
- ELAM
- ELAM1
- ESEL
see all -
数据库链接
- Entrez Gene: 6401 Human
- SwissProt: P16581 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab47348于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Sandwich ELISA |
Use at an assay dependent concentration.
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说明 |
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Sandwich ELISA
Use at an assay dependent concentration. |
数据表及文件
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SDS download
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Datasheet download
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