人CD21 ELISA试剂盒(ab45909)
Key features and details
- Sensitivity: 3 U/ml
- Range: 3.12 U/ml - 100 U/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人CD21 ELISA试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Sample A 8 50.25U/ml 2.24 4.45% Sample B 8 10.44U/ml 0.46 4.4% 批次间 样品 n Mean SD CV% Sample A 8 51.13U/ml 2.29 4.47% Sample B 8 10.76U/ml 0.56 5.2% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 3 U/ml -
范围
3.12 U/ml - 100 U/ml -
检测时间
1h 45m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s CD21 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD21 in Human serum, plasma, buffered solutions and cell culture media.
A monoclonal antibody specific for CD21 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD21 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD21 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD21 present in the samples.
This kit will recognize both endogenous and recombinant Human CD21.
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 2 x 96 tests 1 x 96 tests 1 x 96 tests 10X Standard Diluent Buffer Black 1 x 25ml 1 x 15ml 1 x 15ml 200X Wash Buffer White 2 x 10ml 1 x 10ml 1 x 10ml Biotinylated Antibody Diluent Red 1 x 13ml 1 x 7.5ml 1 x 7.5ml Biotinylated anti-CD21 antibody Red 2 x 400µl 1 x 400µl 1 x 400µl CD21 Microplate (12 x 8 well strips) 1 x 2 units 1 unit 1 unit CD21 Standard Yellow 4 vials 2 vials 2 vials Chromogen TMB Substrate Solution 1 x 24ml 1 x 11ml 1 x 11ml HRP Diluent Red 1 x 23ml 1 x 12ml 1 x 12ml Stop Reagent Black 2 x 11ml 1 x 11ml 1 x 11ml Streptavidin-HRP 4 x 5µl 2 x 5µl 2 x 5µl -
研究领域
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相关性
CD21 also known as complement receptor 2 (CR2), C3d receptor or EBV receptor is a 140 kDa protein. CD21 is a glycosylated type I transmembrane protein consisting of an extracellular face of a series of 15 or 16 CCP domains. CD21 is the receptor for complement components C3d and iC3b as well as the Epstein-Barr virus (EBV) glycoprotein gp350/220. The soluble CD21 (sCD21) was shown to efficiently trigger CD23 signalling pathways in human monocytes. By inducing release of proinflammatory cytokines and upregulating expression of molecules involved in antigen presentation, sCD21 modulates critical monocyte functions that may be relevant to allergic and inflammatory disorders. -
细胞定位
Type 1 membrane protein -
别名
- C3DR
- Complement C3d receptor
- Complement receptor type 2
see all -
数据库链接
- Entrez Gene: 1380 Human
- Omim: 120650 Human
- SwissProt: P20023 Human
图片
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Typical Standard Curve
Representative Standard Curve using ab45909.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab45909 尚未被引用在任何文献中。