人ARP4 ELISA试剂盒(Angiopoietin-Like 4) (ab99974)
Key features and details
- Sensitivity: 20 pg/ml
- Range: 27.4 pg/ml - 20000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人ARP4 ELISA试剂盒(Angiopoietin-Like 4) -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 20 pg/ml -
范围
27.4 pg/ml - 20000 pg/ml -
回收率
100.4 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 117.8 67% - 137% Serum 95.38 85% - 104% Plasma 88.04 74% - 103% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s ARP4 (Angiopoietin-like 4) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human ARP4 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human ARP4 coated on a 96-well plate. Standards and samples are pipetted into the wells and ARP4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human ARP4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ARP4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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说明
Optimisation may be required with urine samples.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 280X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml ARP4 Microplate (12 x 8 well strips) 1 unit Assay Diluent A 1 x 30ml Biotinylated anti-Human ARP4 2 vials Recombinant ARP4 Standard Human (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
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相关性
ARP4 is a fasting-induced adipose factor. It was identified as an adipocytokine up-regulated by fasting, by peroxisome proliferator-activated receptor agonists, and by hypoxia. At the protein level, in human and mouse blood plasma, ARP4 was found to be present both as a native protein and in a truncated form. Differentiation of mouse 3T3-L1 adipocytes was associated with the production of truncated ARP4, whereas in human white adipose tissue and SGBS adipocytes, only the native ARP4 could be detected. Interestingly, the truncated ARP4 was produced by human liver. Experimental data suggest that ARP4 is mainly presented in human blood plasma in a truncated form (ARP4-S2), whose level is increased by fenofibrate treatment. Levels of both truncated and native ARP4 showed marked inter individual variation but were not associated with body mass index and were not influenced by prolonged semistarvation. -
别名
- 425O18 1
- Angiopoietin like 4
- Angiopoietin related protein 4
see all -
数据库链接
- SwissProt: Q9BY76 Human
图片
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Typical Standard Curve
Representative standard curve using ab99974.
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Typical Standard CurveRepresentative standard curve using ab99974.
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab99974 被引用在 9 文献中.
- Wang BD et al. Bevacizumab attenuates osteosarcoma angiogenesis by suppressing MIAT encapsulated by serum-derived extracellular vesicles and facilitating miR-613-mediated GPR158 inhibition. Cell Death Dis 13:272 (2022). PubMed: 35347106
- Tanaka T et al. Association of high cellular expression and plasma concentration of angiopoietin-like 4 with tongue cancer lung metastasis and poor prognosis. Oncol Lett 24:299 (2022). PubMed: 35949602
- Yuan X et al. Gut Microbiota of Chinese Obese Children and Adolescents With and Without Insulin Resistance. Front Endocrinol (Lausanne) 12:636272 (2021). PubMed: 33815293
- Chen S et al. LncRNA CCAL Promotes Angiogenesis Through Regulating the MiR-29b/ANGPTL4 Axis in Osteosarcoma. Cancer Manag Res 12:10521-10530 (2020). PubMed: 33122950
- Chen RM et al. Adropin and glucagon-like peptide-2 are associated with glucose metabolism in obese children. World J Pediatr 15:565-571 (2019). PubMed: 31598832
- Tarhoni I et al. Development of bead based multiplexed immunoassay for evaluation of midkine, syndecan-1, and ANGPTL4 in patient serum. J Immunoassay Immunochem 39:84-98 (2018). PubMed: 29309212
- Frenzel E et al. a1-Antitrypsin Combines with Plasma Fatty Acids and Induces Angiopoietin-like Protein 4 Expression. J Immunol 195:3605-16 (2015). PubMed: 26363050
- Zhao X et al. Clostridium butyricum reduce lipogenesis through bacterial wall components and butyrate. Appl Microbiol Biotechnol 98:7549-57 (2014). PubMed: 24878750
- Florea V et al. c-Myc Is Essential to Prevent Endothelial Pro-Inflammatory Senescent Phenotype. PLoS One 8:e73146 (2013). PubMed: 24039874