人Apo E ELISA试剂盒(ab233623)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 30.34 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Milk, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Apo E ELISA试剂盒
参阅全部 Apolipoprotein E 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Serum 3 1.6% 批次间 样品 n Mean SD CV% Serum 8 9.5% -
样品类型
Milk, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
30.34 pg/ml -
范围
62.5 pg/ml - 4000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Milk 109 95% - 119% Serum 108 89% - 122% Hep Plasma 114 96% - 123% EDTA Plasma 108 100% - 117% Cit plasma 117 106% - 125% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Human Apo E ELISA Kit (ab233623) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Apo E protein in cit plasma, edta plasma, hep plasma, milk, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Apo E with 10.8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Please refer to protocols. -
组件 1 x 96 tests 10X Human Apo E Capture Antibody 1 x 600µl 10X Human Apo E Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml Antibody Diluent 4BI 1 x 6ml Human Apo E Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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功能
Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues. -
组织特异性
Occurs in all lipoprotein fractions in plasma. It constitutes 10-20% of very low density lipoproteins (VLDL) and 1-2% of high density lipoproteins (HDL). APOE is produced in most organs. Significant quantities are produced in liver, brain, spleen, lung, adrenal, ovary, kidney and muscle. -
疾病相关
Defects in APOE are a cause of hyperlipoproteinemia type 3 (HLPP3) [MIM:107741]; also known as familial dysbetalipoproteinemia. Individuals with HLPP3 are clinically characterized by xanthomas, yellowish lipid deposits in the palmar crease, or less specific on tendons and on elbows. The disorder rarely manifests before the third decade in men. In women, it is usually expressed only after the menopause. The vast majority of the patients are homozygous for APOE*2 alleles. More severe cases of HLPP3 have also been observed in individuals heterozygous for rare APOE variants. The influence of APOE on lipid levels is often suggested to have major implications for the risk of coronary artery disease (CAD). Individuals carrying the common APOE*4 variant are at higher risk of CAD.
Genetic variations in APOE are associated with Alzheimer disease type 2 (AD2) [MIM:104310]. It is a late-onset neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death. Note=The APOE*4 allele is genetically associated with the common late onset familial and sporadic forms of Alzheimer disease. Risk for AD increased from 20% to 90% and mean age at onset decreased from 84 to 68 years with increasing number of APOE*4 alleles in 42 families with late onset AD. Thus APOE*4 gene dose is a major risk factor for late onset AD and, in these families, homozygosity for APOE*4 was virtually sufficient to cause AD by age 80. The mechanism by which APOE*4 participates in pathogenesis is not known.
Defects in APOE are a cause of sea-blue histiocyte disease (SBHD) [MIM:269600]; also known as sea-blue histiocytosis. This disorder is characterized by splenomegaly, mild thrombocytopenia and, in the bone marrow, numerous histiocytes containing cytoplasmic granules which stain bright blue with the usual hematologic stains. The syndrome is the consequence of an inherited metabolic defect analogous to Gaucher disease and other sphingolipidoses.
Defects in APOE are a cause of lipoprotein glomerulopathy (LPG) [MIM:611771]. LPG is an uncommon kidney disease characterized by proteinuria, progressive kidney failure, and distinctive lipoprotein thrombi in glomerular capillaries. It mainly affects people of Japanese and Chinese origin. The disorder has rarely been described in Caucasians. -
序列相似性
Belongs to the apolipoprotein A1/A4/E family. -
翻译后修饰
Synthesized with the sialic acid attached by O-glycosidic linkage and is subsequently desialylated in plasma. O-glycosylated with core 1 or possibly core 8 glycans. Thr-307 is a minor glycosylation site compared to Ser-308.
Glycated in plasma VLDL of normal subjects, and of hyperglycemic diabetic patients at a higher level (2-3 fold).
Phosphorylation sites are present in the extracelllular medium. -
细胞定位
Secreted. - Information by UniProt
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别名
- AD2
- Apo-E
- APOE
see all -
数据库链接
- Entrez Gene: 348 Human
- Omim: 107741 Human
- SwissProt: P02649 Human
- Unigene: 654439 Human
相关产品
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Alternative Versions
图片
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Standard curve comparison between Human Apo E SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of Apo E were measured in duplicates, interpolated from the Apo E standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:8000, plasma (citrate) 1:8000, plasma (EDTA) 1:10000, and plasma (heparin) 1:8000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apo E concentration was determined to be 11588.11 ng/mL in serum, 9516.25 pg/mL in plasma (citrate), 14224.65 ng/mL in plasma (EDTA), and 12539.13 ng/mL in plasma (heparin).
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The concentrations of Apo E were measured in duplicates, interpolated from the Apo E standard curve and corrected for sample dilution. Undiluted sample is milk 1:400. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apo E concentration was determined to be 512.93 ng/mL.
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To learn more about the advantages of recombinant antibodies see here.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab233623 被引用在 1 文献中.
- Vogt LM et al. Apolipoprotein E Triggers Complement Activation in Joint Synovial Fluid of Rheumatoid Arthritis Patients by Binding C1q. J Immunol 204:2779-2790 (2020). PubMed: 32253242