Triiodothyronine (T3) ELISA试剂盒(ab108685)
Key features and details
- Sensitivity: 0.05 ng/ml
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
概述
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产品名称
Triiodothyronine (T3) ELISA试剂盒
参阅全部 Triiodothyronine (T3) 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 40 < 10.7% 批次间 样品 n Mean SD CV% Overall 3 < 9.1% -
样品类型
Serum, Hep Plasma, Cit plasma -
检测类型
Competitive -
灵敏度
0.05 ng/ml -
回收率
97.5 %
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实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Triiodothyronine (T3) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of total Triiodothyronine in serum and plasma.
A 96-well plate has been precoated with anti-T3. Samples and the T3-HRP conjugate are added to the wells, where Triiodothyronine in the sample competes with the addedT3-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Triiodothyronine in the sample and the intensity is measured at 450 nm.
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经测试应用
适用于: Competitive ELISAmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 11X T3-HRP Conjugate 1 x 1.4ml 50X Wash Solution 1 x 20ml Anti-T3 IgG Coated Microplate (12 x 8 wells) 1 unit Conjugate Buffer 1 x 12.5ml Cover foils 1 unit Stop Solution 1 x 15ml Strip holder 1 unit T3 Standard 0 - 0.0 ng/mL 1 x 1ml T3 Standard 1 - 0.5 ng/mL 1 x 1ml T3 Standard 2 - 1.0 ng/mL 1 x 1ml T3 Standard 3 - 2.5 ng/mL 1 x 1ml T3 Standard 4 - 5.0 ng/mL 1 x 1ml T3 Standard 5 - 7.5 ng/mL 1 x 1ml TMB Substrate Solution 1 x 15ml -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108685于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Competitive ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Competitive ELISA
Use at an assay dependent concentration. |
图片
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Typical Standard Curve
Representative Standard Curve using ab108685
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab108685 被引用在 4 文献中.
- Zhang M et al. Ageing related thyroid deficiency increases brain-targeted transport of liver-derived ApoE4-laden exosomes leading to cognitive impairment. Cell Death Dis 13:406 (2022). PubMed: 35468877
- Tani N et al. Thyroid-related hormones as potential markers of hypoxia/ischemia. Hum Cell 33:545-558 (2020). PubMed: 32146707
- Gil-Cayuela C et al. Myocardium of patients with dilated cardiomyopathy presents altered expression of genes involved in thyroid hormone biosynthesis. PLoS One 13:e0190987 (2018). PubMed: 29320567
- Frye RE et al. Thyroid dysfunction in children with autism spectrum disorder is associated with folate receptor α autoimmune disorder. J Neuroendocrinol 29:N/A (2017). PubMed: 28199771