Thyroxine (Total T4) ELISA试剂盒(ab178664)
Key features and details
- Sensitivity: 0.004 µg/ml
- Range: 0.044 µg/ml - 0.108 µg/ml
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
概述
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产品名称
Thyroxine (Total T4) ELISA试剂盒
参阅全部 Thyroxine (T4) 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Sera 20 <=8.16% 批次间 样品 n Mean SD CV% Sera 10 <=8.42% -
样品类型
Serum, Hep Plasma, Cit plasma -
检测类型
Competitive -
灵敏度
> 0.004 µg/ml -
范围
0.044 µg/ml - 0.108 µg/ml -
回收率
97.8 %
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实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Thyroxine (T4) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of T4 in serum and plasma.
A 96-well plate has been precoated with anti-Thyroxine (T4) antibodies (solid-phase). Samples and the Thyroxine (T4)-HRP conjugate are added to the wells, where Thyroxine (T4) in the sample competes with the added Thyroxine (T4)-HRP conjugate for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Thyroxine (T4) in the sample and the intensity is measured at 450 nm.
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经测试应用
适用于: Competitive ELISAmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 50X Washing Solution 1 x 20ml Anti-Thyroxine IgG Coated Microplate (12 x 8 wells) 1 unit Conjugate Buffer 1 x 12.5ml Cover Foil 1 unit Stop Solution 1 x 15ml Strip holder 1 unit Thyroxine HRP Conjugate 1 x 1.4ml Thyroxine Standard 0 - 0 µg/mL 1 x 1ml Thyroxine Standard 1 1 x 1ml Thyroxine Standard 2 - 0.050 µg/mL 1 x 1ml Thyroxine Standard 3 - 0.100 µg/mL 1 x 1ml Thyroxine Standard 4 - 0.150 µg/mL 1 x 1ml Thyroxine Standard 5 - 0.250 µg/mL 1 x 1ml TMB Substrate Solution 1 x 15ml -
研究领域
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数据库链接
- SwissProt: P05543 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab178664于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Competitive ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Competitive ELISA
Use at an assay dependent concentration. |
图片
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Typical Standard Curve
Representative standard curve using ab178664.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab178664 被引用在 3 文献中.
- Zhang M et al. Ageing related thyroid deficiency increases brain-targeted transport of liver-derived ApoE4-laden exosomes leading to cognitive impairment. Cell Death Dis 13:406 (2022). PubMed: 35468877
- Gil-Cayuela C et al. Myocardium of patients with dilated cardiomyopathy presents altered expression of genes involved in thyroid hormone biosynthesis. PLoS One 13:e0190987 (2018). PubMed: 29320567
- Moura SL et al. Theoretical and experimental study for the biomimetic recognition of levothyroxine hormone on magnetic molecularly imprinted polymer. Biosens Bioelectron 107:203-210 (2018). PubMed: 29471281