Thromboxane B2 ELISA试剂盒(ab133022)
Key features and details
- Sensitivity: 10.54 pg/ml
- Range: 13.7 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, Plasma, Platelets, Saliva, Serum, Tissue Culture Media, Urine, Whole Blood
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Species independent
概述
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产品名称
Thromboxane B2 ELISA试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Low 24 759pg/ml = 3.6% Medium 24 1283pg/ml = 4% High 24 2605pg/ml = 1.6% 批次间 样品 n Mean SD CV% Low 8 44pg/ml = 7.6% Medium 8 371pg/ml = 3.6% High 8 3053pg/ml = 6.2% -
样品类型
Cell culture supernatant, Saliva, Urine, Serum, Plasma, Platelets, Whole Blood, Cell Lysate, Tissue Culture Media -
检测类型
Competitive -
灵敏度
= 10.54 pg/ml -
范围
13.7 pg/ml - 10000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Saliva = 98.3 % - % Urine = 102.2 % - % Serum = 113 % - % Tissue Culture Media = 104.7 % - % Hep Plasma = 111 % - % -
检测时间
3h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Species independent -
产品概述
Abcam’s Thromboxane B2 (TXB2) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Thromboxane B2 in biological fluids.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-TXB2 antigen and a rabbit polyclonal antibody specific to TXB2. After incubation the excess reagents are washed away and pNpp substrate is added. After a short incubation the enzyme reaction is stopped and a yellow color is generated. The intensity of the yellow coloration is inversely proportional to the amount of TXB2 captured in the plate.
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说明
Thromboxane A2 (TXA2) is involved in platelet aggregation, vasoconstriction and reproductive functions, but has a half life of only 37 seconds under physiological conditions. Thromboxane B2 (TXB2) is the stable product of the non-enzymatic hydration of TXA2, thus the production of TXA2 in vivo is typically monitored by measurement of TXB2 and its metabolite 2,3-dinor TXB2. 11-dehydro-TXB2 is a major metabolite of Thromboxane B2 (TXB2) found in urine and plasma produced by its dehydrogenation by the enzyme 11-OH-dehydrogenase.
Cross Reactivity
Compound Cross Reactivity TXB2 100% 2,3-dinorTXB2 7.1% 11-dehydro TXB2 0.4% 6-keto-PGF1α 0.23% PGD2 <0.1% PGE2 <0.1% PGF1α <0.1% PGF2α <0.1% -
经测试应用
适用于: Competitive ELISAmore details -
平台
Microplate
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer Concentrate 1 x 27ml Assay Buffer 1 x 27ml Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit Plate Sealer 1 unit pNpp Substrate 1 x 20ml Rabbit Polyclonal Thromboxane B2 Antibody 1 x 5ml Stop Solution 1 x 5ml Thromboxane B2 Alkaline Phosphatase Conjugate 1 x 5ml Thromboxane B2 Standard 1 x 500µl -
研究领域
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别名
- T box 2
- T box transcription factor TBX2
- T-box protein 2
see all
相关产品
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133022于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Competitive ELISA |
Use at an assay dependent concentration.
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说明 |
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Competitive ELISA
Use at an assay dependent concentration. |
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab133022 被引用在 6 文献中.
- Panova-Noeva M et al. Variation of platelet function in clinical phenotypes of acute venous thromboembolism - Results from the GMP-VTE project. J Thromb Haemost 20:705-715 (2022). PubMed: 34784445
- Yin B et al. The antithrombosis effect of dehydroandrographolide succinate: in vitro and in vivo studies. Pharm Biol 60:175-184 (2022). PubMed: 35014931
- Gál K et al. Monitoring and recovery of hyperglycaemia-induced endothelial dysfunction with rheopheresis in diabetic lower extremity ulceration with hyperviscosity. Diab Vasc Dis Res 19:14791641221131788 (2022). PubMed: 36357361
- Zhang Y et al. Is it safe to take Radix Salvia Miltiorrhiza - Radix Pueraria Lobate product with warfarin and aspirin? A pilot study in healthy human subjects. J Ethnopharmacol 262:113151 (2020). PubMed: 32736050
- Panova-Noeva M et al. Comprehensive platelet phenotyping supports the role of platelets in the pathogenesis of acute venous thromboembolism - results from clinical observation studies. EBioMedicine 60:102978 (2020). PubMed: 32920367
- Ahmad AA et al. Sex differences in the role of phospholipase A2 -dependent arachidonic acid pathway in the perivascular adipose tissue function in pigs. J Physiol 595:6623-6634 (2017). PubMed: 28877347