STAT5 A/B pY694/699 ELISA试剂盒(ab176656)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 5 µg/ml
- Range: 10 µg/ml - 1000 µg/ml
- Sample type: Adherent cells, Cell culture extracts, Suspension cells, Tissue Homogenate
- Detection method: Colorimetric
- Assay type: Semi-quantitative
- Reacts with: Human
概述
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产品名称
STAT5 A/B pY694/699 ELISA试剂盒
参阅全部 STAT5 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% A431 extract 6 2.8% 批次间 样品 n Mean SD CV% A431 extract 3 4% -
样品类型
Cell culture extracts, Adherent cells, Suspension cells, Tissue Homogenate -
检测类型
Semi-quantitative -
灵敏度
< 5 µg/ml -
范围
10 µg/ml - 1000 µg/ml -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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产品概述
Abcam’s STAT5 A/B (pY694/699) in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the semi-quantitative measurement of STAT5 A/B (pY694/699) protein in Human cells.
The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm
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说明
Abcam’s STAT5 A/B (pY694/699) SimpleStep ELISA kit (ab176656) has been re-developed with new detector antibody. From February 18, 2016 the kit will be delivered with a new detector total STAT5 antibody. This change was necessary because of a decrease in the performance of the polyclonal STAT5 antibody that was previously used for detection in recent production lots. We have identified a new antibody to use in the SimpleStep ELISA platform that provides higher performance.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
经测试应用
适用于: ELISAmore details -
平台
Microplate
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 1 x 96 tests 10X Wash Buffer PT 1 x 15ml 1 x 15ml 50X Cell Extraction Enhancer Solution 1 x 1ml 1 x 1ml 5X Cell Extraction Buffer PTR 1 x 12ml 1 x 12ml Lyophilized STAT5 A/B Control Lysate 1 vial 1 vial Plate Seal 1 unit 1 unit SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 1 unit STAT5 A/B (pY694/699) Capture Antibody 1 x 3ml 1 x 3ml STAT5 A/B (pY694/699) Detector Antibody 1 x 3ml 1 x 3ml Stop Solution 1 x 12ml 1 x 12ml TMB Substrate 1 x 12ml 1 x 12ml -
研究领域
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功能
Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription. -
序列相似性
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
翻译后修饰
Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. -
细胞定位
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation. - Information by UniProt
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别名
- MGF
- Signal transducer and activator of transcription 5A
- Signal Transducer and Activator of Transcription 5B
see all -
数据库链接
- Entrez Gene: 6777 Human
- Entrez Gene: 6776 Human
- Entrez Gene: 20851 Mouse
- Entrez Gene: 20850 Mouse
- Entrez Gene: 25126 Rat
- Entrez Gene: 24918 Rat
- Omim: 601511 Human
- Omim: 604260 Human
see all
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab176656于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
ELISA
Use at an assay dependent concentration. |
图片
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Typical cell lysate dilution series
Example of a typical STAT5 A/B (pY694/699) cell lysate dilution series. Background-subtracted data values (mean +/- SD) are graphed.
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Induction of STAT5 A/B (pY694/699) phosphorylation in A431 cells in response to EGF treatment.A431 cells were cultured in 96-well tissue culture plates and treated (15 min) with a dose-range of EGF before cell lysis. Data from quadruplicate measurements of STAT5 A/B (pY694/699) are plotted and compared against Total STAT5 A/B levels.
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Cell line analysis for Total STAT5 A/BCell line analysis for Total STAT5 A/B from 100 µg/mL preparations of cell extracts. Data from triplicate measurements (mean +/- SD) are plotted and compared to 1X Cell Extraction Buffer PTR (zero).
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Sandwich ELISA - STAT5 A/B pY694/699 ELISA Kit (ab176656)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide. -
Sandwich ELISA - STAT5 A/B pY694/699 ELISA Kit (ab176656)To learn more about the advantages of SimpleStep ELISA® kits see here.
数据表及文件
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SDS download
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Datasheet download
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