AB126428

STAT4 (Tyr693) In-Cell ELISA试剂盒

Name: STAT4 (Tyr693) In-Cell ELISA试剂盒 (ab126428)| Abcam中文官网
Brand: Abcam Limited
SKU: AB126428
Price: 7672 CNY
Availability: InStock

STAT4 (Tyr693) In-Cell ELISA Kit

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STAT4 (Tyr693) In-Cell ELISA Kit is a Cell-based ELISA for the measurement of STAT4 (Tyr693) In-Cell in Human, Mouse in Cell Samples samples.

查看别名

Signal transducer and activator of transcription 4, STAT4

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In-Cell ELISA - STAT4 (Tyr693) In-Cell ELISA Kit (AB126428)

In-Cell ELISA

Unknown

In-Cell ELISA - STAT4 (Tyr693) In-Cell ELISA Kit (AB126428)

A431 cells were stimulated by different concentrations of recombinant human EGF for 10 minutes at 37°C

Supplier Data

Western blot - STAT4 (Tyr693) In-Cell ELISA Kit (AB126428)

Western blot analysis of extracts from 100 ng/ml hEGF treated A431 cells. Phospho-STAT4 (Tyr693) and STAT4 antibodies were used in both detection assays.

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关键信息

检测方法

Colorimetric

样品类型

Adherent cells

反应种属

Mouse, Human

检测类型

Cell-based

检测时间

5h 10m

检测平台

Microplate

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

ab126428 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of STAT4 (Tyr693) phosphorylation and screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured human and mouse cell lines. By determining STAT4 protein phosphorylation in your experimental model system, you can verify pathway activation in your cell lines without spending excess time and effort in preparing cell lysate and performing an analysis of Western Blot.

In the STAT4 (Tyr693) In-Cell ELISA Kit, cells are seeded into a 96 well tissue culture plate. The cells are fixed after various treatments, inhibitors or activators. After blocking, Anti Phospho-STAT4 (Tyr693) or Anti-STAT4 (primary antibody) is pipetted into the wells and incubated. The wells are washed, and HRP-conjugated anti-Mouse IgG or HRP-conjugated anti-rabbit IgG (secondary antibody) is added to the wells. The wells are washed again, a TMB substrate solution is added to the wells and color develops in proportion to the amount of protein. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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规格

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性能和储存信息

运输条件

Blue Ice

储存信息

-20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The Signal Transducer and Activator of Transcription 4 (STAT4) is a critical member of the STAT protein family. It weighs approximately 85 kDa. STAT4 primarily functions as a transcription factor activated by phosphorylation through Janus kinases (JAKs) in response to cytokine signaling. This protein facilitates the translocation into the nucleus where it promotes the transcription of specific genes. STAT4 expression occurs mainly in T cells and natural killer (NK) cells indicating its role in the immune response.

Biological function summary

STAT4 is integral to the differentiation of naïve T cells into T helper 1 (Th1) cells. These Th1 cells play a central role in orchestrating immune responses against intracellular pathogens. STAT4 activation requires two distinct steps involving JAK kinases and subsequent binding to DNA regulatory elements of target genes. STAT4 does not function as part of a large protein complex but rather operates as a dimer following phosphorylation.

Pathways

STAT4 participates strongly in the JAK-STAT signaling pathway fundamental for immune cell maturation and response. The JAK-STAT pathway regulates several biological processes including proliferation and inflammation. STAT4 works closely with STAT1 in promoting Th1 differentiation while also interacting with other proteins like IL-12 to modulate immune activity. These interactions emphasize its influence in immune responses and could affect inflammatory pathways.

STAT4 exhibits associations with autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosus. Aberrant STAT4 activity can lead to dysregulated immune responses making it a significant target in these disorders. In the context of rheumatoid arthritis altered STAT4 signaling enhances inflammation through its connection with other immune proteins like IL-23. Understanding STAT4's role in these diseases may offer avenues for therapeutic interventions and better management of immune-related conditions.

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产品实验方案

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靶点信息

Transcriptional regulator mainly expressed in hematopoietic cells that plays a critical role in cellular growth, differentiation and immune response (PubMed : 10961885, PubMed : 37256972, PubMed : 8943379). Plays a key role in the differentiation of T-helper 1 cells and the production of interferon-gamma (PubMed : 12213961, PubMed : 35614130). Also participates in multiple neutrophil functions including chemotaxis and production of the neutrophil extracellular traps (By similarity). After IL12 binding to its receptor IL12RB2, STAT4 interacts with the intracellular domain of IL12RB2 and becomes tyrosine phosphorylated (PubMed : 10415122, PubMed : 7638186). Phosphorylated STAT4 then homodimerizes and migrates to the nucleus where it can recognize STAT target sequences present in IL12 responsive genes. Although IL12 appears to be the predominant activating signal, STAT4 can also be phosphorylated and activated in response to IFN-gamma stimulation via JAK1 and TYK2 and in response to different interleukins including IL23, IL2 and IL35 (PubMed : 11114383, PubMed : 34508746). Transcription activation of IFN-gamma gene is mediated by interaction with JUN that forms a complex that efficiently interacts with the AP-1-related sequence of the IFN-gamma promoter (By similarity). In response to IFN-alpha/beta signaling, acts as a transcriptional repressor and suppresses IL5 and IL13 mRNA expression during response to T-cell receptor (TCR) activation (PubMed : 26990433).

See full target information STAT4

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STAT4 (Tyr693) In-Cell ELISA试剂盒

STAT4 (Tyr693) In-Cell ELISA Kit

In-Cell ELISA - STAT4 (Tyr693) In-Cell ELISA Kit (AB126428)

Western blot - STAT4 (Tyr693) In-Cell ELISA Kit (AB126428)