STAT1 (pS727) + total STAT1 ELISA试剂盒(ab126455)
Key features and details
- Sample type: Cell Lysate
- Detection method: Colorimetric
- Assay type: Semi-quantitative
- Reacts with: Mouse, Human
概述
-
产品名称
STAT1 (pS727) + total STAT1 ELISA试剂盒
参阅全部 STAT1 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell Lysate -
检测类型
Semi-quantitative -
检测时间
5h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse, Human -
产品概述
ab126455 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates. By determining phosphorylated STAT1 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blot analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-STAT1 (Ser727) and total STAT1 (help normalize the results of phospho-STAT1 from different cell lysate being compared). An anti-STAT1 (Ser727) (half plate, red marker on left side) and anti-total STAT1 antibody (half plate, black marker on right side) has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated (left side) and total (right side) STAT1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated STAT1 is used to detect phosphorylated or total STAT1. After washing away unbound antibody, HRP-conjugated Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of STAT1 (Ser727) or total STAT1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
-
说明
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
经测试应用
适用于: ELISAmore details -
平台
Microplate
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 2X Cell Lysis Buffer 1 x 5ml 500x HRP-conjugated anti-rabbit IgG concentrate 1 x 25µl 5X Assay Diluent 1 x 15ml Detection Antibody anti-pan STAT1 2 vials Detection Antibody anti-phospho STAT1 (Ser727) 1 vial HRP-Streptavidin Concentrate 2 x 200µl Positive Control: lyophilized powder from A431 cell lysate 1 vial STAT1 Microplate with anti-pan STAT1 antibody 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
-
功能
Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. -
疾病相关
Note=STAT1 deficiency results in impaired immune response leading to severe mycobacterial and viral diseases. In the case of complete deficiency, patients can die of viral disease.
Defects in STAT1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance. -
序列相似性
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
翻译后修饰
Phosphorylated on tyrosine and serine residues in response to IFN-alpha, IFN-gamma, PDGF and EGF. Phosphorylation on Tyr-701 (lacking in beta form) by JAK promotes dimerization and subsequent translocation to the nucleus. Phosphorylation on Ser-727 by several kinases including MAPK14, ERK1/2 and CAMKII on IFN-gamma stimulation, regulates STAT1 transcriptional activity. Phosphorylation on Ser-727 promotes sumoylation though increasing interaction with PIAS. Phosphorylation on Ser-727 by PKCdelta induces apoptosis in response to DNA-damaging agents.
Sumoylated by SUMO1, SUMO2 and SUMO3. Sumoylation is enhanced by IFN-gamma-induced phosphorylation on Ser-727, and by interaction with PIAS proteins. Enhances the transactivation activity.
ISGylated. -
细胞定位
Cytoplasm. Nucleus. Translocated into the nucleus in response to IFN-gamma-induced tyrosine phosphorylation and dimerization. - Information by UniProt
-
别名
- Signal transducer and activator of transcription 1 91kD
- CANDF7
- DKFZp686B04100
see all -
数据库链接
- Entrez Gene: 6772 Human
- Entrez Gene: 20846 Mouse
- Omim: 600555 Human
- SwissProt: P42224 Human
- SwissProt: P42225 Mouse
- Unigene: 642990 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab126455于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
ELISA
Use at an assay dependent concentration. |
图片
-
Western blot - STAT1 (pS727) + total STAT1 ELISA Kit (ab126455)The A431 cells were treated with 100 ng/ml recombinant human EGF for 20 minutes. Serial dilutions of lysates were analyzed by Western blot. Immunoblots were incubated with anti-phospho-STAT1 (Ser727).
-
Sandwich ELISA - STAT1 (pS727) + total STAT1 ELISA Kit (ab126455)The A431 cells were treated with 100 ng/ml recombinant human EGF for 20 minutes. Serial dilutions of lysates were analyzed in this ELISA. -
Western blot - STAT1 (pS727) + total STAT1 ELISA Kit (ab126455)A431 cells were treated or untreated with 100 ng/ml recombinant human EGF for 10 min. Cell lysates were analyzed by Western Blot. -
Sandwich ELISA - STAT1 (pS727) + total STAT1 ELISA Kit (ab126455)A431 cells were treated or untreated with 100 ng/ml recombinant human EGF for 10 min. Cell lysates were analyzed using this phosphoELISA. -
Sandwich ELISA - STAT1 (pS727) + total STAT1 ELISA Kit (ab126455)A431 cells were treated with recombinant human EGF at 37°C for 20 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab126455 被引用在 2 文献中.
- Kim GO et al. Protective Effects of Cirsilineol against Lipopolysaccharide-Induced Inflammation; Insights into HO-1, COX-2, and iNOS Modulation. Int J Mol Sci 24:N/A (2023). PubMed: 37239882
- Kroetz DN et al. Type I Interferon Induced Epigenetic Regulation of Macrophages Suppresses Innate and Adaptive Immunity in Acute Respiratory Viral Infection. PLoS Pathog 11:e1005338 (2015). PubMed: 26709698