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AB270552

SimpleStep ELISA® Custom ELISA试剂盒

SimpleStep ELISA® Custom ELISA Kit

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SimpleStep ELISA® Custom ELISA Kit allows you to develop your own SimpleStep ELISA® using any antibody pair.

- No plate coating needed
- Possibility to measure different target on the same plate
- All components included, just add the antibody pair
- Streamline your ELISA® to a single-wash format if desired
4 Images
ELISA - SimpleStep ELISA® Custom ELISA Kit (AB270552)
  • ELISA

Supplier Data

ELISA - SimpleStep ELISA® Custom ELISA Kit (AB270552)
ELISA - SimpleStep ELISA® Custom ELISA Kit (AB270552)
  • ELISA

Supplier Data

ELISA - SimpleStep ELISA® Custom ELISA Kit (AB270552)

Schematic representation of how the kit works

ELISA - SimpleStep ELISA® Custom ELISA Kit (AB270552)
  • ELISA

Supplier Data

ELISA - SimpleStep ELISA® Custom ELISA Kit (AB270552)

SimpleStep ELISA® Custom ELISA Kit and conventional passive coating approach were compared. With ab270552, 10x less capture antibody can be used without affecting the assay performance.

ELISA - SimpleStep ELISA® Custom ELISA Kit (AB270552)
  • ELISA

Supplier Data

ELISA - SimpleStep ELISA® Custom ELISA Kit (AB270552)

Other solution-phase binding ELISA formats typically use biotin to immobilize immunocomplexes. These can constrain the sample types that can be used with the assay. In contrast, ab270552 has been specifically designed to be used with most sample matrices without interference.

关键信息

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搭配使用产品

Reagents, Controls & Accessories

AB206978

Pre-Coated 96 Well Microplate (12 x 8 well strips) SimpleStep ELISA®

SimpleStep

ELISA - Pre-Coated 96 Well Microplate (12 x 8 well strips) SimpleStep ELISA® (AB206978)

  • 0
  • 0
Reagents, Controls & Accessories

AB203359

Pre-coated 384 well Microplate SimpleStep ELISA®

SimpleStep

ELISA - Pre-coated 384 well Microplate SimpleStep ELISA® (AB203359)

  • 5
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Reagents, Controls & Accessories

AB206977

10X Wash Buffer PT

SimpleStep

ELISA - 10X Wash Buffer PT (AB206977)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

SimpleStep ELISA® Custom ELISA Kit (ab270552) is the latest cutting-edge technology with a built-in system for antibody labeling. Built on Abcam’s patented CaptSureTM technology this system enables the rapid and easy development of in-house ELISAs using any antibody pair, while eliminating the need to perform plate coating procedures.

It is powered by the combination of proprietary technologies, enabling you to reduce the amount of capture antibody required, measure different targets on the same plate with different assay antibody pairs, and streamline your ELISA to a single-wash format if desired.

The capture antibody of interest is quickly and easily conjugated with the CaptSureTM Peptide supplied within the kit. The interaction between the antibody coated on the plate and the CaptSureTM Peptide immobilizes the immune-complex.

The detection antibody is similarly conjugated with HRP using the Lightning-Link® HRP conjugation reagents, also supplied in the kit. Thus, with minimal hands-on time, you can set up both of your antibodies to ensure the best performance of your ELISA.

This kit has ready-to-go assay plates, pre-coated with antibody, which are specific for the CaptSureTM Peptide.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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规格

{ "values": { "5x96Tests": { "sellingSize": "5 x 96 Tests", "publicAssetCode":"ab270552-5x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"50X Cell Extraction Enhancer Solution (ab270697)", "number":"AB270552-CMP20" }, { "size":"1 x 200 µL", "name":"Modifier reagent", "number":"AB270552-CMP14" }, { "size":"1 x 1 Unit", "name":"10X Wash Buffer PT (ab270697)", "number":"AB270552-CMP18" }, { "size":"1 x 1 Unit", "name":"Stop Solution (ab270697)", "number":"AB270552-CMP11" }, { "size":"1 x 1 Unit", "name":"5X Cell Extraction Buffer PTR (ab270697)", "number":"AB270552-CMP19" }, { "size":"2 x 1 Unit", "name":"Sample Dilution Buffer (ab270697)", "number":"AB270552-CMP09" }, { "size":"1 x 1 Vial", "name":"CaptSure™ Quencher Reagent (ab270690)", "number":"AB270552-CMP15" }, { "size":"1 x 1 Vial", "name":"CaptSure™ Modifier Reagent (ab270690)", "number":"AB270552-CMP17" }, { "size":"1 x 1 Unit", "name":"HRP-Labeled Antibody Dilution Buffer (ab270697)", "number":"AB270552-CMP05" }, { "size":"1 x 1 Unit", "name":"Peptide tagged Antibody Dilution Buffer (ab270697)", "number":"AB270552-CMP04" }, { "size":"1 x 1 Unit", "name":"Detection Reagent (ab270697)", "number":"AB270552-CMP03" }, { "size":"1 x 200 µL", "name":"Quencher reagent", "number":"AB270552-CMP02" }, { "size":"1 x 5 Tests", "name":"Assay Plates (ab275264)", "number":"AB270552-CMP01" }, { "size":"3 x 1 Vial", "name":"CaptSure™ Peptide (ab270690)", "number":"AB270552-CMP16" }, { "size":"3 x 100 µg", "name":"HRP mix", "number":"AB270552-CMP13" } ] }, "5x96Tests": { "sellingSize": "5 x 96 Tests", "publicAssetCode":"ab270552-5x96Tests", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"Modifier reagent", "number":"AB270552-CMP14", "productcode":"" }, { "size":"1 x 1 Vial", "name":"CaptSure™ Quencher Reagent (ab270690)", "number":"AB270552-CMP08", "productcode":"" }, { "size":"1 x 5 Tests", "name":"Assay Plates (ab275264)", "number":"AB270552-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"CaptSure™ Modifier Reagent (ab270690)", "number":"AB270552-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Stop Solution (ab270697)", "number":"AB270552-CMP11", "productcode":"" }, { "size":"2 x 1 Unit", "name":"Sample Dilution Buffer (ab270697)", "number":"AB270552-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"5X Cell Extraction Buffer PTR (ab270697)", "number":"AB270552-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"10X Wash Buffer PT (ab270697)", "number":"AB270552-CMP12", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Peptide tagged Antibody Dilution Buffer (ab270697)", "number":"AB270552-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"HRP-Labeled Antibody Dilution Buffer (ab270697)", "number":"AB270552-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"Quencher reagent", "number":"AB270552-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Detection Reagent (ab270697)", "number":"AB270552-CMP03", "productcode":"" }, { "size":"3 x 100 µg", "name":"HRP mix", "number":"AB270552-CMP13", "productcode":"" }, { "size":"3 x 1 Vial", "name":"CaptSure™ Peptide (ab270690)", "number":"AB270552-CMP06", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Ambient - Cannot Ship with Ice
推荐的短期储存条件
Multi
推荐的长期储存条件
Multi
储存信息
Please refer to protocols
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产品实验方案

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靶点信息

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 21:114 PubMed33596916

2021

Targeting SNHG3/miR-186-5p reverses the increased m6A level caused by platinum treatment through regulating METTL3 in esophageal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mingxin Zhang,Minghua Bai,Li Wang,Ning Lu,Jia Wang,Rong Yan,Manli Cui,Honglin Yan,Lingmin Zhang
View all publications
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Abcam Product Promise

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详情请参阅我们的条款与条件。

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