Serotonin ELISA试剂盒(ab133053)
Key features and details
- Sensitivity: 0.293 ng/ml
- Range: 0.49 ng/ml - 500 ng/ml
- Sample type: Plasma, Platelets, Serum, Urine
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Species independent
概述
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产品名称
Serotonin ELISA试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% 346.1 ng/ml 20 4.2% 53.8 ng/ml 20 5.8% 13.5 ng/ml 20 11% 批次间 样品 n Mean SD CV% 358.0 ng/ml 16.2% 53.9 ng/ml 18.4% 13.2 ng/ml 12.7% -
样品类型
Urine, Serum, Plasma, Platelets -
检测类型
Competitive -
灵敏度
0.293 ng/ml -
范围
0.49 ng/ml - 500 ng/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Urine = 101 91% - 107% Serum = 91 83% - 95% Platelets = 107 106% - 109% Cit plasma = 95 83% - 102% -
检测时间
3h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Species independent -
产品概述
Serotonin ELISA kit is a competitive ELISA kit designed for the accurate quantitative measurement of Serotonin in Human Serum, Platelets, Plasma and Urine.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated- Serotonin antigen and a polyclonal rabbit antibody specific to Serotonin. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Serotonin captured in the plate.
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说明
Serotonin (5-hydroxytryptamine, 5-HT) is a monoamine found in the central nervous system, gastrointestinal tract, and blood with broad physiological functions as a neurotransmitter, in gastric motility, hemostasis, and cardiovascular integrity. Defects in serotonin signaling have been linked to a large number of complex behavioral disorders in humans, including anxiety and depression, autism, and eating disorders, and to neurodegenerative disorders such as Parkinson’s. Platelets serve as the major reservoir of serotonin in the bloodstream. When activated, platelets release serotonin into the bloodstream where it acts as a powerful vasoconstrictor. Treatment with Selective Serotonin Uptake Inhibitors (SSRIs) dramatically reduces platelet serotonin concentrations, and altered levels of serotonin in the circulatory system are implicated in such diverse conditions such as asthma, liver cirrhosis and carcinoid tumors.
Cross reactivities:
Compound Cross Reactivity N-Acetyl Serotonin 17% 5-Hydroxy-L-tryptophan 0.4% Tryptamine 0.1% 5-Hydroxyindoleacetic acid 0.03% Melatonin 0.01% Tyramine <0.004% Tryptophan <0.004% -
经测试应用
适用于: Competitive ELISAmore details -
平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer Concentrate 1 x 27ml Assay Buffer 1 x 27ml Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit Plate Sealer 2 units pNpp Substrate 1 x 20ml Serotonin Alkaline Phosphatase Conjugate 2 x 150ng Serotonin Antibody 2 vials Serotonin Standard 2 x 250ng Stop Solution 1 x 5ml -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133053于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Competitive ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Competitive ELISA
Use at an assay dependent concentration. |
图片
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ab133053 Serotonin ELISA Kit
Serotonin measured (averages of undil., 1:3, 1:9 & 1:27) in biological fluids (Neuro2a an Growth med. averaged from 1:2 and 1:4) showing quantity (ng) per mL of tested sample.
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Typical Standard CurveRepresentative Standard Curve using ab133053.
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Typical Parrallelism GraphDose-response curves from Human platelets and Human serum diluted into assay buffer were compared to the Serotonin standard curve. The parallel response indicates the standard effectively mimics the native protein.
数据表及文件
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SDS download
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Datasheet download
文献 (32)
ab133053 被引用在 32 文献中.
- Almobarak B et al. Exposure to nonanoic acid alters small intestinal neuroendocrine tumor phenotype. BMC Cancer 23:267 (2023). PubMed: 36959559
- Jeong JJ et al. Multi-strain probiotics alleviate loperamide-induced constipation by adjusting the microbiome, serotonin, and short-chain fatty acids in rats. Front Microbiol 14:1174968 (2023). PubMed: 37333632
- Huang J et al. Mesenchymal stromal cells alleviate depressive and anxiety-like behaviors via a lung vagal-to-brain axis in male mice. Nat Commun 14:7406 (2023). PubMed: 37973914
- Kim KH & Lee KA Metabolic Rewiring by Human Placenta-Derived Mesenchymal Stem Cell Therapy Promotes Rejuvenation in Aged Female Rats. Int J Mol Sci 23:N/A (2022). PubMed: 35008991
- Wauquier F et al. Circulating Human Serum Metabolites Derived from the Intake of a Saffron Extract (Safr'InsideTM) Protect Neurons from Oxidative Stress: Consideration for Depressive Disorders. Nutrients 14:N/A (2022). PubMed: 35406124