大鼠Prolactin R ELISA试剂盒(PRLR) (ab113351)
Key features and details
- Sensitivity: 0.4 ng/ml
- Range: 0.512 ng/ml - 50 ng/ml
- Sample type: Cell culture supernatant
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
概述
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产品名称
大鼠Prolactin R ELISA试剂盒(PRLR)
参阅全部 Prolactin Receptor/PRL-R 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant -
检测类型
Sandwich (quantitative) -
灵敏度
< 0.4 ng/ml -
范围
0.512 ng/ml - 50 ng/ml -
回收率
100 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 125.4 117% - 141% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Rat -
产品概述
Abcam’s Prolactin R Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat Prolactin R in cell culture supernatants.
This assay employs an antibody specific for rat Prolactin R coated on a 96-well plate. Standards and samples are pipetted into the wells and Prolactin R present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat Prolactin R antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Prolactin R bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect the endogenous Rat Prolactin R in normal serum with ab113351, only in serum spiked with Rat Prolactin R.
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说明
Note: very low signal were obtained in normal plasma.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Biotinylated anti-rat Prolactin R 2 vials Prolactin R Microplate (12 x 8 wells) 1 unit Recombinant rat Prolactin R Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
This is a receptor for the anterior pituitary hormone prolactin (PRL). Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling. -
组织特异性
Expressed in breast, placenta, kidney, liver and pancreas. -
序列相似性
Belongs to the type I cytokine receptor family. Type 1 subfamily.
Contains 2 fibronectin type-III domains. -
结构域
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
细胞定位
Secreted and Membrane. - Information by UniProt
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别名
- AI987712
- CLONE SPM213
- CPRLP
see all -
数据库链接
- Entrez Gene: 24684 Rat
- SwissProt: P05710 Rat
- Unigene: 9757 Rat
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab113351于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Typical data
Representative standard curve using ab113351
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab113351 被引用在 2 文献中.
- Salihu M et al. 6-Gingerol-rich fraction from Zingiber officinale ameliorates carbendazim-induced endocrine disruption and toxicity in testes and epididymis of rats. Andrologia 49:N/A (2017). PubMed: 27546232
- Adedara IA et al. Municipal Landfill Leachate-Induced Testicular Oxidative Damage is Associated with Biometal Accumulation and Endocrine Disruption in Rats. Arch Environ Contam Toxicol N/A:N/A (2014). PubMed: 25179371