AB157740

大鼠Plasminogen ELISA试剂盒

Name: 大鼠Plasminogen ELISA试剂盒 (ab157740)| Abcam中文官网
Brand: Abcam Limited
SKU: AB157740

Rat Plasminogen ELISA Kit

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(1 Publication)

Rat Plasminogen ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat Plasminogen in Rat in Biofluids samples.

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Plasminogen, Plg

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Sandwich ELISA - Rat Plasminogen ELISA Kit (AB157740)

sELISA

Supplier Data

Sandwich ELISA - Rat Plasminogen ELISA Kit (AB157740)

Representative standard curve using ab157740 Plasminogen Rat ELISA Kit

关键信息

检测方法

Colorimetric

样品类型

Plasma, Serum

反应种属

Rat

检测类型

Sandwich (quantitative)

灵敏度

= 3.24 ng/mL

范围

3.13 - 200 ng/mL

检测平台

Microplate

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Abcam's Plasminogen Rat ELISA Kit is a two-site enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Plasminogen levels in rat serum and plasma.

In this assay the Plasminogen present in samples reacts with the anti-Plasminogen antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Plasminogen antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Plasminogen. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Plasminogen in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Plasminogen in the test sample. The quantity of Plasminogen in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

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精确度

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]

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回收率

[ { "sample": "Serum", "range": null, "average": "> 85" } ]

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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab157740-1x96Tests", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"5X Diluent Concentrate", "number":"AB157740-CMP07", "productcode":"" }, { "size":"1 x 150 µL", "name":"100X HRP-conjugated anti-rat Plasminogen antibody", "number":"AB157740-CMP05", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Wash Buffer Concentrate", "number":"AB157740-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Chromogen Substrate Solution", "number":"AB157740-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB157740-CMP03", "productcode":"" }, { "size":"12 x 8 Test", "name":"Rat Plasminogen ELISA Microplate", "number":"AB157740-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Rat Plasminogen Calibrator (lyophilized)", "number":"AB157740-CMP01", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

+4°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Plasminogen also known as PLG is an important protein with a molecular weight of approximately 92 kDa. Plasminogen is a zymogen which means it is an inactive precursor that requires activation to function. It is produced primarily in the liver and circulates in blood plasma. Its expression occurs mainly in the liver but you can detect it in various tissues including the kidney. Plasminogen binds to tissue surfaces and undergoes transformation into plasmin an active enzyme that plays significant roles in many biological processes.

Biological function summary

Plasminogen plays a central role in fibrinolysis which is the breakdown of fibrin clots. This process is essential in wound healing and maintaining normal blood flow. Plasminogen once activated to plasmin acts by degrading fibrin a major component of blood clots. The protein forms part of the fibrinolytic system along with other components like tissue plasminogen activator (tPA). Together they ensure that any clots forming within blood vessels are regulated and dissolved as necessary preventing blockages.

Pathways

Plasminogen is deeply involved in the coagulation and fibrinolytic systems. The conversion of plasminogen to plasmin is a critical step in these pathways requiring activators such as urokinase. Plasminogen has interactions with proteins like alpha-2-antiplasmin and thrombin which help modulate its activity. These interactions ensure a careful balance is maintained between clot formation and dissolution which is vital for normal bodily function.

Plasminogen deficiency is linked with conditions like ligneous conjunctivitis a rare disorder characterized by membranous lesions mainly in the eyes. Reduced plasminogen activity can lead to excessive clot formation contributing to thrombosis risk. Plasminogen's interaction with plasmin and its deficiency can also associate with angioedema a condition that causes sudden swelling in the deeper layers of skin and tissues. Understanding the pathways and the proteins involved in plasminogen's function presents therapeutic potential in managing these disorders.

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产品实验方案

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靶点信息

Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells (By similarity).. Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo.

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of thrombosis and thrombolysis 45:77-87 PubMed29168147

2017

Generation and characterization of tissue-type plasminogen activator transgenic rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yusuke Ito,Kengo Noguchi,Yoshiyuki Morishima,Kyoji Yamaguchi

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大鼠Plasminogen ELISA试剂盒

Rat Plasminogen ELISA Kit

Sandwich ELISA - Rat Plasminogen ELISA Kit (AB157740)