AB201283

大鼠PAI1 ELISA试剂盒(SERPINE1)

Rat PAI1 ELISA Kit (SERPINE1)

Recombinant
SimpleStep
了解详情

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(3 Publications)

Rat PAI1 ELISA Kit (SERPINE1) is a single-wash 90-min Simplestep used to quantify Rat PAI1 (SERPINE1) with a sensitivity of 159 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

Pai1, Planh1, Serpine1, Plasminogen activator inhibitor 1, PAI, PAI-1, Endothelial plasminogen activator inhibitor, Serpin E1

3 Images

Sandwich ELISA - Rat PAI1 ELISA Kit (SERPINE1) (AB201283)

sELISA

Supplier Data

Sandwich ELISA - Rat PAI1 ELISA Kit (SERPINE1) (AB201283)

Interpolated concentrations of PAI1 in rat serum.

The concentrations of PAI1 were measured in duplicate and interpolated from the PAI1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PAI1 concentration was determined to be 5,897 pg/mL in mouse serum.

sELISA

Supplier Data

Sandwich ELISA - Rat PAI1 ELISA Kit (SERPINE1) (AB201283)

Example of PAI1 standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Rat PAI1 ELISA Kit (SERPINE1) (AB201283)

Interpolated Concentration of PAI1 in rat serum, plasma-citrate, and urine.

The concentrations of PAI1 were measured in duplicate and interpolated from the PAI1 standard curve and corrected for sample dilution. The Interpolated dilution factor corrected values are plotted (mean+/- SD, n=2).

关键信息

检测方法

Colorimetric

样品类型

Urine, Citrate plasma, Cell culture supernatant, Serum

反应种属

Mouse, Rat

检测类型

Sandwich

灵敏度

= 159 pg/mL

范围

298 - 5000 pg/mL

检测时间

1h 30m

检测平台

Microplate

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Rat PAI1 ELISA Kit (SERPINE1) (ab201283) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PAI1 (SERPINE1) protein in cell culture supernatant, cit plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat PAI1 (SERPINE1) with 159 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Plasminogen activator inhibitor-1 (PAI1 or Serpin E1) is encoded by the Serpine1 gene. PAI1 is a serine protease inhibitor with a major role in the regulation of fibrinolysis. PAI1 is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), both of which are activators of plasminogen. PAI1 is a secreted protein produced by the endothelium as well as adipose tissue.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Rat Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" }, { "reproducibilityType": "Intra", "sample": "Rat Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.6" } ]

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回收率

[ { "sample": "Serum", "range": "94 - 105 %", "average": "= 100" }, { "sample": "Urine", "range": "69 - 88 %", "average": "= 80" }, { "sample": "Citrate plasma", "range": "84 - 87 %", "average": "= 85" }, { "sample": "Cell culture media", "range": "98 - 102 %", "average": "= 99" }, { "sample": "serum free media", "range": "91 - 98 %", "average": "= 95" } ]

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规格

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab201283-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB201283-CMP04" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB201283-CMP10" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB201283-CMP05" }, { "size":"1 x 6000 µL", "name":"10X Rat PAI1 Capture Antibody", "number":"AB201283-CMP07" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB201283-CMP02" }, { "size":"2 x 10 Vial", "name":"Rat PAI1 Lyophilized Recombinant Protein", "number":"AB201283-CMP01" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB201283-CMP06" }, { "size":"1 x 6000 µL", "name":"10X Rat PAI1 Detector Antibody", "number":"AB201283-CMP08" }, { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB201283-CMP09" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB201283-CMP03" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab201283-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB201283-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB201283-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB201283-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB201283-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB201283-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB201283-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat PAI1 Capture Antibody", "number":"AB201283-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rat PAI1 Lyophilized Recombinant Protein", "number":"AB201283-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB201283-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat PAI1 Detector Antibody", "number":"AB201283-CMP08", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

+4°C

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产品实验方案

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靶点信息

Serine protease inhibitor. Inhibits TMPRSS7. Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots. As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading. Acts as a regulator of cell migration, independently of its role as protease inhibitor. It is required for stimulation of keratinocyte migration during cutaneous injury repair. It is involved in cellular and replicative senescence (By similarity). Plays a role in alveolar type 2 cells senescence in the lung (By similarity). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis (By similarity).

See full target information Serpine1

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Sarcoidosis, vasculitis, and diffuse lung diseases : official journal of WASOG 40:e2023036 PubMed37712376

2023

Protective efficacy of pirfenidone in rats with pulmonary fibrosis induced by bleomycin.

Applications

Unspecified application

Species

Unspecified reactive species

Baris Demirkol,Sule Gul,Mustafa Cörtük,Neslihan Akanıl Fener,Eminegül Yavuzsan,Ramazan Eren,Kursad Nuri Baydili,Mustafa Baki Çekmen,Erdogan Cetinkaya

Bone & joint research 10:41-50 PubMed33448865

2021

Fibrinolysis as a target to enhance osteoporotic fracture healing by vibration therapy in a metaphyseal fracture model.

Applications

Unspecified application

Species

Unspecified reactive species

Ronald Man Yeung Wong,Victoria Man Huen Choy,Jie Li,Tsz Kiu Li,Yu Ning Chim,Meng Chen Michelle Li,Jack Chun Yiu Cheng,Kwok-Sui Leung,Simon Kwoon-Ho Chow,Wing Hoi Cheung

Journal of thrombosis and thrombolysis 45:77-87 PubMed29168147

2017

Generation and characterization of tissue-type plasminogen activator transgenic rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yusuke Ito,Kengo Noguchi,Yoshiyuki Morishima,Kyoji Yamaguchi

View all publications

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websiteProtocolBooklet

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大鼠PAI1 ELISA试剂盒(SERPINE1)

Rat PAI1 ELISA Kit (SERPINE1)

Sandwich ELISA - Rat PAI1 ELISA Kit (SERPINE1) (AB201283)

Sandwich ELISA - Rat PAI1 ELISA Kit (SERPINE1) (AB201283)

Sandwich ELISA - Rat PAI1 ELISA Kit (SERPINE1) (AB201283)

关键信息

检测方法

样品类型

反应种属

检测类型

灵敏度

范围

检测时间

检测平台