大鼠Laminin ELISA试剂盒(ab119573)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 156 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
概述
-
产品名称
大鼠Laminin ELISA试剂盒
参阅全部 Laminin beta 2 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts -
检测类型
Sandwich (quantitative) -
灵敏度
< 10 pg/ml -
范围
156 pg/ml - 10000 pg/ml -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Rat -
产品概述
Abcam’s rat Laminin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of rat Laminin in cell culture supernatants, serum, cell lysates and plasma (heparin, EDTA).
A Laminin specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards (form rat sarcoma basement membrane) and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for Laminin is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the rat Laminin amount of sample captured in plate.
-
经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 标识符 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml Anti-rat Laminin Antibody Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti-rat Laminin antibody 1 x 100µl Lyophilized rat Laminin standard 2 vials Plate Seal 1 x 4 units Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
研究领域
- Information by UniProt
-
别名
- Lamb2
- LAMB2_HUMAN
- Laminin B1s chain
see all -
数据库链接
- Entrez Gene: 25473 Rat
- SwissProt: P15800 Rat
- Unigene: 774 Rat
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119573于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
-
Typical Standard Curve
Representative Standard Curve using ab119573
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab119573 尚未被引用在任何文献中。