AB190545

大鼠L-FABP ELISA试剂盒

Name: 大鼠L-FABP ELISA试剂盒 (ab190545)| Abcam中文官网
Brand: Abcam Limited
SKU: AB190545
Price: 7452 CNY
Availability: InStock

Rat L-FABP ELISA Kit

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Rat L-FABP ELISA Kit is a Sandwich ELISA for the measurement of Rat L-FABP in Rat in Biofluids samples.

查看别名

Fatty acid-binding protein 1, Liver-type fatty acid-binding protein, Squalene- and sterol-carrier protein, Z-protein, p14, L-FABP, SCP, Fabp1

1 Images

Sandwich ELISA - Rat L-FABP ELISA Kit (AB190545)

sELISA

Supplier Data

Sandwich ELISA - Rat L-FABP ELISA Kit (AB190545)

Representative standard curve using ab190545 L-FABP Rat ELISA Kit.

关键信息

检测方法

Colorimetric

样品类型

Plasma, Serum

反应种属

Rat

检测类型

Sandwich

灵敏度

= 1.278 ng/mL

范围

3.125 - 200 ng/mL

检测平台

Microplate

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Abcam's L-FABP Rat ELISA Kit (ab190545) is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of L-FABP in rat serum and plasma.

In this assay the L-FABP present in samples reacts with the anti-L-FABP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-L-FABP antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound L-FABP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of L-FABP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of L-FABP in the test sample. The quantity of L-FABP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

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精确度

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]

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回收率

[ { "sample": "Serum", "range": null, "average": "> 85" } ]

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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab190545-1x96Tests", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"20X Wash Buffer Concentrate", "number":"AB190545-CMP07", "productcode":"" }, { "size":"1 x 60 mL", "name":"1X Diluent Concentrate", "number":"AB190545-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"L-FABP Rat HRP Conjugate", "number":"AB190545-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"L-FABP Rat Calibrator (Lyophilized)", "number":"AB190545-CMP04", "productcode":"" }, { "size":"1 x 96 Test", "name":"L-FABP Rat Antibody coated microwells", "number":"AB190545-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Chromogen Substrate Solution", "number":"AB190545-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB190545-CMP01", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

Please refer to protocols

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Liver fatty acid binding protein (liver FABP) also known as L-FABP or FABP1 is a small protein with a mass of approximately 14 kilodaltons. It functions mainly in the liver where it binds free fatty acids and other lipophilic substances facilitating their transport within cells. This protein is highly expressed in hepatocytes and also found in the small intestine and kidneys. Its role in binding fatty acids positions it as an important mediator in lipid metabolism making it of interest in a variety of metabolic studies.

Biological function summary

Liver FABP is essential for maintaining lipid homeostasis within cells. It is not part of a larger complex but acts by itself to regulate the intracellular concentration of lipids and protect cells from lipotoxicity. By sequestering fatty acids it prevents these molecules from disrupting cellular membranes or signaling pathways. Liver FABP also participates in the uptake transport and metabolic conversion of fatty acids and their metabolites influencing energy homeostasis and other vital processes.

Pathways

Liver FABP plays a significant role in the fatty acid metabolism and peroxisome proliferator-activated receptor (PPAR) signaling pathways. It acts by modulating the availability of lipid ligands necessary for PPAR activation linking it functionally to these nuclear receptors that control gene expression involved in lipid and glucose metabolism. Liver FABP's association with proteins like FABP2 and FABP4 within these pathways provides insight into its broader metabolic network highlighting its interactions in fatty acid transport and metabolism.

Liver FABP shows a strong connection to metabolic conditions particularly non-alcoholic fatty liver disease (NAFLD) and type 2 diabetes. Its dysregulation is often observed in these disorders which include altered lipid profiles and insulin resistance. Liver FABP is also linked to certain forms of cancer with aberrant expression levels found in some tumor types. The interactions of liver FABP with proteins such as FABP5 in these diseases suggest a potential role in the pathogenesis of metabolic disorders and tumor development making it a candidate for further research and therapeutic targeting.

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产品实验方案

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靶点信息

Plays a role in lipoprotein-mediated cholesterol uptake in hepatocytes (By similarity). Binds cholesterol (PubMed : 25732850). Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport (By similarity).

See full target information Fatty acid-binding protein, liver

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websiteProtocolBooklet

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大鼠L-FABP ELISA试剂盒

Rat L-FABP ELISA Kit

Sandwich ELISA - Rat L-FABP ELISA Kit (AB190545)