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大鼠KIM-1 TIM-1 ELISA试剂盒(ab119597)

Submit a review Q&A (6)References (17)
Typical Standard Curve

    Key features and details

    • Sensitivity: 2 pg/ml
    • Range: 31.2 pg/ml - 2000 pg/ml
    • Sample type: Cell culture supernatant, Plasma, Serum, Urine
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Rat

    概述

    • 产品名称

      大鼠KIM-1 TIM-1 ELISA试剂盒
      参阅全部 TIM 1 试剂盒

    • 检测方法

      Colorimetric

    • 样品类型

      Cell culture supernatant, Urine, Serum, Plasma

    • 检测类型

      Sandwich (quantitative)

    • 灵敏度

      < 2 pg/ml

    • 范围

      31.2 pg/ml - 2000 pg/ml

    • 实验步骤

      Multiple steps standard assay

    • 种属反应性

      与反应: Rat

    • 产品概述

      Abcam’s rat KIM-1 (TIM-1) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of rat KIM-1 (TIM-1) in cell culture supernatants, tissue homogenates, serum, plasma (heparin, EDTA) and urine.

      A KIM-1 (TIM-1) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for KIM-1 (TIM-1) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the rat KIM-1 (TIM-1) amount of sample captured in plate.

    • 经测试应用

      适用于: Sandwich ELISAmore details

    • 平台

      Microplate

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab119597于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Sandwich ELISA
    Use at an assay dependent concentration.
    说明
    Sandwich ELISA
    Use at an assay dependent concentration.

    图片

    • Typical Standard Curve
      Typical Standard Curve

      Representaive Standard Curve using ab119597

    文献 (17)

    发表研究结果有使用 ab119597？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab119597 被引用在 17 文献中.

    • Younis NS Myrrh Essential Oil Mitigates Renal Ischemia/Reperfusion-Induced Injury. Curr Issues Mol Biol 45:1183-1196 (2023). PubMed: 36826023
    • Meneses ME  et al. Effects of Mexican Ganoderma lucidum extracts on liver, kidney, and the gut microbiota of Wistar rats: A repeated dose oral toxicity study. PLoS One 18:e0283605 (2023). PubMed: 37022999
    • Mohamed ME  et al. The Protective Effect of Anethole against Renal Ischemia/Reperfusion: The Role of the TLR2,4/MYD88/NF?B Pathway. Antioxidants (Basel) 11:N/A (2022). PubMed: 35326185
    • Guo J  et al. Ginsenoside Rg1 ameliorates sepsis-induced acute kidney injury by inhibiting ferroptosis in renal tubular epithelial cells. J Leukoc Biol 112:1065-1077 (2022). PubMed: 35774015
    • Mohamed ME  et al. Renal Ischemia/Reperfusion Mitigation via Geraniol: The Role of Nrf-2/HO-1/NQO-1 and TLR2,4/MYD88/NFκB Pathway. Antioxidants (Basel) 11:N/A (2022). PubMed: 36009287
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-6 of 6 Abreviews or Q&A

    Question

    As I’m not familiar with these items, I tried to find the ab codes through your site.
    For the items I found a relative code I have update the list.
    For those I don’t have a reference code, this is because either no products matches or all available products reacts for humans when we need them for pigs.
    So please check the list again and advice.
    https://www.abcam.com/Procalcitonin-Human-ELISA-Kit-ab100630.html Porcine Procalcitonin, PCT ELISA kit 96 Tests
    https://www.abcam.com/IL6-Pig-ELISA-Kit-1-x-96-Well-Plate-ab100755.html Porcine IL-6 Quantikine ELISA kit 96 Tests
    https://www.abcam.com/TNF-alpha-Porcine-ELISA-Kit-ab100756.html Porcine TNF-alpha/ TNFSF1A Quantikine ELISA kit 96 Tests
    https://www.abcam.com/Cortisol-Human-ELISA-Kit-ab108674.html Porcine Cortisol ELISA kit 96 Tests ? (Is it the same for humans and pigs?)
    Porcine Noradrenalin ELISA kit 96 Tests (No product match)
    Porcine Adrenaline ELISA kit 96 Tests (No product match)
    https://www.abcam.com/Albumin-Swine-ELISA-Kit-ab108794.html Porcine Serum Cystatin C ELISA Kit 96 Tests
    Porcine Urinary Cystatin C ELISA Kit 96 Tests (Only for humans)
    https://www.abcam.com/IL8-Pig-ELISA-Kit-ab113352.html Porcine Serum NGAL (Neutrophil Gelatinase-Associated Lipocalin) ELISA Kit 96 Tests
    Porcine Urinary NGAL (Neutrophil Gelatinase-Associated Lipocalin) ELISA Kit 96 Tests (Only for humans)
    Porcine Urinary KIM-1 (Kidney Injury Molecule -1) ELISA Kit 96 Tests (Only for humans)
    https://www.abcam.com/IL8-Pig-ELISA-Kit-ab113352.html Porcine Urinary IL-18 (Interleukin -18) ELISA Kit 96 Tests
    Porcine Urinary NAG (N-Acetyl-β-D-Glucosaminidase) ELISA Kit96 Tests (Only for humans)
    https://www.abcam.com/IFN-gamma-Pig-ELISA-Kit-ab113353.html Porcine Urinary L-FABP (Liver-type Fatty-Acid Binding Protein) ELISA Kit 96 Tests

    Read More
    Answer

    Thank you for contacting us.


    To let you know, we are currently investigating the Porcine (pig) species, cross-reactivity information for following kits ab119602, ab113326, ab119600, ab119601, ab119590 and ab119589. We will send you the details soon.

    The other kits ab100755, ab100756, ab108674, ab108794, ab113352, ab113353 are OK to use with Porcine samples and are guaranteed.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

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    Question

    Inquiry: Hi, We're planning to use the KIM-1 TIM-1 Rat ELISA kit to measure this in rat tissue samples. We were looking at the sample preparation section and wondered if PBS and homogenisation would be sufficient to disrupt kidney tissue enough to get these molecules into suspension?? Look forward to hearing back from you soon. Kind regards

    Read More
    Answer

    I can confirm that PBS and homogenization will be absolutely fine and can be used flawlessly with the kit. Using tissue lysis buffer/solution is even better but PBS alone should work perfectly well.

    Read More

    Question

    I've been using this kit with rat urine. Is this kit pH dependent? Should we be adjusting the pH of our samples to ensure accuracy?

    Read More
    Answer

    The pH of rat urine is close to 7.0, and there is no need to adjust the pH before use with the kit.

    Read More

    Question

    I found you have the Rat Kim-1 ELISA kit. Do you have the Rat Kim-1 antibody for immuohistochemistry?

    Read More
    Answer

    Thank you for contacting us.
    We have several TIM1 antibodies (TIM1 is a synonym for KIM1).
    Two of these antibodies were tested in IHC with paraffin sections with human and mouse, but not rat.
    If you like to try using these antibodies in rat, you could use our testing discount program for untested species.
    For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
    The testing discount program is for customers who like to use an antibody/protein/kit on an untested species/application. You would purchase the antibody/protein/kit at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody/protein at the full price (100%) of the antibody/protein you have tested, or a full price discount for the amount paid for the kit. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.
    This program applies to ELISA kits and other kits we offer.
    Please let me know if you would be interested in receiving a discount code.
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    As I’m not familiar with these items, I tried to find the ab codes through your site.
    For the items I found a relative code I have update the list.
    For those I don’t have a reference code, this is because either no products matches or all available products reacts for humans when we need them for pigs.
    So please check the list again and advice.
    https://www.abcam.com/Procalcitonin-Human-ELISA-Kit-ab100630.html Porcine Procalcitonin, PCT ELISA kit 96 Tests
    https://www.abcam.com/IL6-Pig-ELISA-Kit-1-x-96-Well-Plate-ab100755.html Porcine IL-6 Quantikine ELISA kit 96 Tests
    https://www.abcam.com/TNF-alpha-Porcine-ELISA-Kit-ab100756.html Porcine TNF-alpha/ TNFSF1A Quantikine ELISA kit 96 Tests
    https://www.abcam.com/Cortisol-Human-ELISA-Kit-ab108674.html Porcine Cortisol ELISA kit 96 Tests ? (Is it the same for humans and pigs?)
    Porcine Noradrenalin ELISA kit 96 Tests (No product match)
    Porcine Adrenaline ELISA kit 96 Tests (No product match)
    https://www.abcam.com/Albumin-Swine-ELISA-Kit-ab108794.html Porcine Serum Cystatin C ELISA Kit 96 Tests
    Porcine Urinary Cystatin C ELISA Kit 96 Tests (Only for humans)
    https://www.abcam.com/IL8-Pig-ELISA-Kit-ab113352.html Porcine Serum NGAL (Neutrophil Gelatinase-Associated Lipocalin) ELISA Kit 96 Tests
    Porcine Urinary NGAL (Neutrophil Gelatinase-Associated Lipocalin) ELISA Kit 96 Tests (Only for humans)
    Porcine Urinary KIM-1 (Kidney Injury Molecule -1) ELISA Kit 96 Tests (Only for humans)
    https://www.abcam.com/IL8-Pig-ELISA-Kit-ab113352.html Porcine Urinary IL-18 (Interleukin -18) ELISA Kit 96 Tests
    Porcine Urinary NAG (N-Acetyl-β-D-Glucosaminidase) ELISA Kit96 Tests (Only for humans)
    https://www.abcam.com/IFN-gamma-Pig-ELISA-Kit-ab113353.html Porcine Urinary L-FABP (Liver-type Fatty-Acid Binding Protein) ELISA Kit 96 Tests

    Read More
    Answer

    I have received information regarding

    ab119589 Cystatin C Human ELISA Kit
    ab119590 Cystatin C Mouse ELISA Kit
    ab119596 KIM1 Mouse ELISA Kit
    ab119597 KIM1 Rat ELISA Kit
    ab119601 Lipocalin-2 / NGAL Mouse ELISA Kit
    ab119601 Lipocalin-2 / NGAL Mouse ELISA Kit

    these has not been tested with Swine or pig samples so we are unable to confirm the species cross reactivity. It mean only ab100755, ab100756, ab108674, ab108794, ab113352, ab113353 are OK to use with Porcine samples and are guaranteed. Would you like to have a quote for these antibodies.

    I hope this info will be helpful. Should you require more information please do not hesitate to contact me.

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    Question

    Thank you for your prompt reply. This is the information I needed. I am also looking to purchase a similar style ELISA kit for rat kidney injury molecule-1 (Rat KIM-1). Does your company sell that item?

    Read More
    Answer

    Thank you for contacting us. It is my pleasure to help.

    We do offer a KIM1 rat ELISA kit as well. This kit has a similar system containing one plate made of twelve 8 well strips. The product number is ab119597 and may be found at the following link:

    https://www.abcam.com/KIM1-Rat-ELISA-Kit-ab119597.html

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

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