Rat IL-1Ra ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat IL-1Ra with a sensitivity of 6.95 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Il-1ra, Il1rn, Interleukin-1 receptor antagonist protein, IL-1RN, IL-1ra, IRAP, IL1 inhibitor
- sELISA
Supplier Data
Sandwich ELISA - Rat IL-1Ra ELISA Kit (AB282875)
Representative image of Rat IL-1Ra ELISA Kit (ab282875)
Components shown from left to right :
- Sample diluent NS
- 10X Rat IL-1Ra Capture Antibody
- 10X Rat IL-1Ra Detector Antibody
- Rat IL-1Ra Lyophilised Purified Protein (2 vials)
- 50X Cell Extraction Enhancer Solution (ab193971)
- Stop Solution
- TMB solution
- 10X Wash Buffer PT (ab206977)
- Antibody Diluent 5BR
SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.
Plate Seals - shown underneath bottles / vials.
Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.
Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit
- sELISA
Supplier Data
Sandwich ELISA - Rat IL-1Ra ELISA Kit (AB282875)
Interpolated concentrations of native IL-1Ra in rat cell culture supernatant samples.
The concentrations of IL-1Ra were measured in duplicates, interpolated from the IL-1Ra standard curves and corrected for sample dilution. Undiluted samples are as follows : lung supernatant 25% and spleen supernatant 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-1Ra concentration was determined to be 891.1 pg/mL in lung supernatant and 3,708 pg/mL in spleen supernatant 25%.
- sELISA
Supplier Data
Sandwich ELISA - Rat IL-1Ra ELISA Kit (AB282875)
Example of rat IL-1Ra standard curve in Sample Diluent NS + 1X Enhancer.
The IL-1Ra standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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产品详情
Rat IL-1Ra ELISA kit (ab282875) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Rat IL-1Ra protein in rat cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat IL-1Ra with 6.95 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Interleukin-1 receptor antagonist (IL-1Ra), encoded by the IL1RN gene, is a cell surface receptor and a member of the interleukin-1 cytokine family. IL-1Ra inhibits the activity of IL-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. However, IL-1Ra has no IL-1 like activity. Studies have shown the absence of IL-1Ra allows unopposed action of IL-1, resulting in life-threating systemic inflammation with skin and bone involvement thus suggesting IL-1Ra to play a role in modulating pro-inflammatory responses.
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