大鼠IgG ELISA试剂盒- 30 minutes (ab273153)
Key features and details
- Sensitivity: 4 ng/ml
- Range: 16 ng/ml - 2000 ng/ml
- Sample type: Ascites Fluid, Cell culture supernatant
- Detection method: Colorimetric
- Assay type: Quantitative
概述
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产品名称
大鼠IgG ELISA试剂盒- 30 minutes
参阅全部 IgG 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% A 1/10 10 5.18µg/ml 2.94 % B 1/10 10 2.39µg/ml 3.48 % C 1/10 10 1.34µg/ml 4.61 % 批次间 样品 n Mean SD CV% D 1/100 30 3.2 % D 1/400 30 3.59 % D 1/6400 30 3.13 % -
样品类型
Cell culture supernatant, Ascites Fluid -
检测类型
Quantitative -
灵敏度
4 ng/ml -
范围
16 ng/ml - 2000 ng/ml -
实验步骤
One step assay -
产品概述
The Rat IgG ELISA Kit (ab273153) provides a rapid and easy method (one step ELISA) for the quantitative determination of rat IgG in cell culture supernatant and rat ascitic fluid.
A polyclonal antibody specific to rat IgG (H+L) is pre-coated onto microwells. Samples and standards are pipetted into microwells and rat IgG present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-rat IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of rat IgG in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
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经测试应用
适用于: ELISAmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10 x 96 tests Detection antibody 1 x 12ml 1 x 120ml Pre-coated microwells strips 1 x 96 tests 10 x 96 tests Rat IgG standard 0.0 ng/ml 1 x 0.3ml 1 x 1ml Rat IgG standard 1000 ng/ml 1 x 0.3ml 1 x 1ml Rat IgG standard 125 ng/ml 1 x 0.3ml 1 x 1ml Rat IgG standard 16 ng/ml 1 x 0.3ml 1 x 1ml Rat IgG standard 2000 ng/ml 1 x 0.3ml 1 x 1ml Rat IgG standard 250 ng/ml 1 x 0.3ml 1 x 1ml Rat IgG standard 500 ng/ml 1 x 0.3ml 1 x 1ml Sample Diluent 1 x 30ml 1 x 500ml Stop solution 1 x 12ml 1 x 120ml TMB Substrate 1 x 12ml 1 x 120ml -
研究领域
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细胞定位
Secreted -
别名
- Ig gamma 1 chain C region
- Ig gamma 2 chain C region
- Ig gamma 3 chain C region
see all
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273153于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
ELISA
Use at an assay dependent concentration. |
图片
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Rat Immunoglobulin G Quantification Kit standard curve
Example standard curve.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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