大鼠IFN gamma ELISA试剂盒(ab113349)
Key features and details
- Sensitivity: 100 pg/ml
- Range: 123 pg/ml - 30000 pg/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
概述
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产品名称
大鼠IFN gamma ELISA试剂盒
参阅全部 Interferon gamma 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 100 pg/ml -
范围
123 pg/ml - 30000 pg/ml -
回收率
98 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 99.29 89% - 111% Plasma 92.63 78% - 104% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Rat -
产品概述
Abcam’s IFN gamma Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat IFN gamma in plasma and cell culture supernatants. (Rat IFN gamma concentration in normal plasma is pretty low, it may not be detected in this assay).
This assay employs an antibody specific for IFN gamma coated on a 96-well plate. Standards and samples are pipetted into the wells and IFN gamma present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat IFN gamma antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IFN gamma bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect the endogenous Rat IFN-gamma in normal serum with ab113349, only in serum spiked with Rat IFN-gamma.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Rat IFN Gamma 2 vials IFN gamma Microplate (12 x 8 wells) 1 unit Recombinant rat IFN gamma Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. -
组织特异性
Released primarily from activated T lymphocytes. -
疾病相关
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. -
序列相似性
Belongs to the type II (or gamma) interferon family. -
翻译后修饰
Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. -
细胞定位
Secreted. - Information by UniProt
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别名
- IF 1
- IFG
- IFI
see all -
数据库链接
- Entrez Gene: 25712 Rat
- SwissProt: P01581 Rat
- Unigene: 10795 Rat
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab113349于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Typical data B
Representative standard curve using ab113349
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Typical data ARepresentative standard curve using ab113349
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab113349 被引用在 3 文献中.
- Paul S et al. Reverse translating SULT1A1, a potential biomarker in roentgenographically tested rat model of rapid HAPE induction. Life Sci 229:132-138 (2019). PubMed: 31100325
- Wu DM et al. Effect of microRNA-186 on oxidative stress injury of neuron by targeting interleukin 2 through the janus kinase-signal transducer and activator of transcription pathway in a rat model of Alzheimer's disease. J Cell Physiol 233:9488-9502 (2018). PubMed: 29995978
- Sun Y et al. Reduction of Uterine Perfusion Pressure Induced Redistribution of Endothelin Receptor Type-B Between the Intima and Media Contributes to the Pathogenesis of Pregnancy-Induced Hypertension. Cell Physiol Biochem 44:1715-1725 (2017). PubMed: 29216636