大鼠Haptoglobin ELISA试剂盒(ab108857)
Key features and details
- Sensitivity: 0.56 µg/ml
- Range: 1.875 µg/ml - 120 µg/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Rat
概述
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产品名称
大鼠Haptoglobin ELISA试剂盒
参阅全部 Haptoglobin 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 5.6% 批次间 样品 n Mean SD CV% Overall 10.4% -
样品类型
Serum, Plasma -
检测类型
Competitive -
灵敏度
= 0.56 µg/ml -
范围
1.875 µg/ml - 120 µg/ml -
回收率
99 %
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检测时间
3h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Rat -
产品概述
Abcam’s Haptoglobin Rat in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Haptoglobin in plasma and serum.
A Haptoglobin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Haptoglobin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of haptoglobin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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经测试应用
适用于: Competitive ELISAmore details -
平台
Microplate
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 1 x 30ml 1X Biotinylated Haptoglobulin (Lyophilized) 1 vial Chromogen Substrate 1 x 7ml Rat Haptoglobulin Microplate (12 x 8 well strips) 1 unit Rat Haptoglobulin Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究领域
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功能
As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens. -
组织特异性
Expressed by the liver and secreted in plasma. -
疾病相关
Anhaptoglobinemia -
序列相似性
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 2 Sushi (CCP/SCR) domains. -
细胞定位
Secreted. - Information by UniProt
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别名
- Binding peptide
- BP
- Haptoglobin alpha chain
see all -
数据库链接
- Entrez Gene: 24464 Rat
- SwissProt: P06866 Rat
- Unigene: 10950 Rat
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108857于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Competitive ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Competitive ELISA
Use at an assay dependent concentration. |
图片
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Typical Standard Curve
Representative Standard Curve using ab108857.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab108857 被引用在 1 文献中.
- Sanchez TW et al. Maternal plasma proteomics in a rat model of pregnancy complications reveals immune and pro-coagulant gene pathway activation. Am J Reprod Immunol 83:e13205 (2020). PubMed: 31677200