JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB108850

大鼠Fibronectin ELISA试剂盒

Rat Fibronectin ELISA Kit

Be the first to review this product! Submit a review

|

(5 Publications)

Rat Fibronectin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat Fibronectin in Rat in Biofluids samples.

查看别名

Fibronectin, FN, Fn1

2 Images
Sandwich ELISA - Rat Fibronectin ELISA Kit (AB108850)
  • sELISA

Lab

Sandwich ELISA - Rat Fibronectin ELISA Kit (AB108850)

Fibronectin measured in various samples showing quantity (microgram) per mL of tested sample

Sandwich ELISA - Rat Fibronectin ELISA Kit (AB108850)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Fibronectin ELISA Kit (AB108850)

Representative Standard Curve using ab108850.

关键信息

检测方法

Colorimetric

样品类型

Urine, Plasma, Serum

反应种属

Rat

检测类型

Sandwich (quantitative)

灵敏度

= 69 pg/mL

范围

0.391 - 6.25 ng/mL

检测时间

4h

检测平台

Microplate

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

产品详情

Abcam's Fibronectin Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Fibronectin in plasma, serum, and urine.

A Fibronectin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Fibronectin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Fibronectin captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

style
left-column

精确度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.8" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" } ]
style
left-column

规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108850-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108850-CMP04", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108850-CMP07", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108850-CMP09", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Rat Fibronectin Standard", "number":"AB108850-CMP06", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Rat Fibronectin Antibody", "number":"AB108850-CMP02", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108850-CMP03", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108850-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Rat Fibronectin Microplate (12 x 8 well strips)", "number":"AB108850-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108850-CMP01", "productcode":"" } ] } } }
style
left-column

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
Multi
储存信息
Please refer to protocols
style
left-column
style
left-column

产品实验方案

style
left-column

靶点信息

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (By similarity). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts (By similarity).. Anastellin. Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.. Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5 : ITGB1 integrin receptor signaling.
See full target information Fn1
style
left-column

文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Food science & nutrition 11:1718-1727 PubMed37051364

2023

Effects of on hepatic and renal extracellular compounds turnover under diabetes in rat.

Applications

Unspecified application

Species

Unspecified reactive species

Maha Sayari,Houcine Dab,Mounira Tlili,Lazhar Zourgui,Abdelmajid Khabir

Experimental and therapeutic medicine 22:1017 PubMed34373703

2021

Ellagic acid inhibits high glucose-induced injury in rat mesangial cells via the PI3K/Akt/FOXO3a signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Lin,Guojian Liu,Xiaowen Kang,Ping Guo,Yu Shang,Ruomei Du,Xiyue Wang,Liting Chen,Rui Yue,Fanwu Kong,Qihan Zhu

International urology and nephrology 53:1247-1254 PubMed33942213

2021

Vitamin D protects glomerular mesangial cells from high glucose-induced injury by repressing JAK/STAT signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yiya Yang,Yuting Lei,Yumei Liang,Shuangshuang Fu,Congjun Yang,Kanghan Liu,Yinyin Chen

Frontiers in cellular and infection microbiology 9:466 PubMed31998665

2020

Comparative Analysis of the Host Response in a Rat Model of Deep-Partial and Full-Thickness Burn Wounds With Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Alan J Weaver,Kenneth S Brandenburg,Brian W Smith,Kai P Leung

Molecular medicine reports 16:7297-7304 PubMed28944869

2017

Promoting potential of adipose derived stem cells on peripheral nerve regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayan Guo,Shu Guo,Yuxin Wang,Yanqiu Yu
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB285314

Rat Collagen Type I ELISA Kit

Sandwich ELISA - Rat Collagen Type I ELISA Kit (AB285314)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Assay Kits

AB275897

Rat CTGF ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Rat CTGF ELISA Kit (AB275897)

  • 0
  • 0
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com