大鼠Ferritin ELISA试剂盒(ab157732)
Key features and details
- Sensitivity: 2.7695 ng/ml
- Range: 12.5 ng/ml - 400 ng/ml
- Sample type: Other biological fluids, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
概述
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产品名称
大鼠Ferritin ELISA试剂盒
参阅全部 Ferritin Light Chain 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall < 10% 批次间 样品 n Mean SD CV% Overall < 10% -
样品类型
Serum, Plasma, Other biological fluids -
检测类型
Sandwich (quantitative) -
灵敏度
2.7695 ng/ml -
范围
12.5 ng/ml - 400 ng/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum > 85 % - % -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Rat -
产品概述
Abcam's Ferritin Rat ELISA Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for the quantitative measurement of ferritin in rat biological samples.
In this assay the ferritin present in samples reacts with the anti-Ferritin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Ferritin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Ferritin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Ferritin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Ferritin in the test sample. The quantity of Ferritin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 100X HRP-conjugated anti-rat Ferritin antibody 1 x 150µl 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml Chromogen Substrate Solution 1 x 12ml Rat Ferritin Calibrator (Lyophilized) 1 vial Rat Ferritin ELISA Microplate 1 unit Stop Solution 1 x 12ml -
研究领域
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功能
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. -
疾病相关
Defects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene.
Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels. -
序列相似性
Belongs to the ferritin family.
Contains 1 ferritin-like diiron domain. - Information by UniProt
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别名
- Ferritin L chain
- Ferritin L subunit
- Ferritin light chain
see all -
数据库链接
- Entrez Gene: 29292 Rat
- SwissProt: P02793 Rat
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab157732于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Standard CurveRepresentative standard curve using ab157732 Ferritin Rat ELISA Kit.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab157732 被引用在 6 文献中.
- Kwon JH et al. Intravenous iron injection does not impair liver function in a rat model of cirrhosis. Ann Palliat Med 11:1687-1699 (2022). PubMed: 35016521
- Hlaing CB et al. Assessment of Systemic Toxicity, Genotoxicity, and Early Phase Hepatocarcinogenicity of Iron (III)-Tannic Acid Nanoparticles in Rats. Nanomaterials (Basel) 12:N/A (2022). PubMed: 35407158
- Urso K et al. Iron Protein Succinylate in the Management of Iron Deficiency Anemia: A Comparative Study with Ferrous Sulphate at Low and High Therapeutic Doses. Nutrients 13:N/A (2021). PubMed: 33802720
- Mehanna ET et al. Anti-Oxidant and Anti-Inflammatory Effects of Lipopolysaccharide from Rhodobacter sphaeroides against Ethanol-Induced Liver and Kidney Toxicity in Experimental Rats. Molecules 26:N/A (2021). PubMed: 34946518
- Kang H et al. Renal clearable nanochelators for iron overload therapy. Nat Commun 10:5134 (2019). PubMed: 31723130
- Woodman AG et al. Modest and Severe Maternal Iron Deficiency in Pregnancy are Associated with Fetal Anaemia and Organ-Specific Hypoxia in Rats. Sci Rep 7:46573 (2017). PubMed: 28440316