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AB245706

大鼠Fas ELISA试剂盒

Rat Fas ELISA Kit

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Rat Fas ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat Fas with a sensitivity of 26.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

CD95, Apt1, Tnfrsf6, Fas, Tumor necrosis factor receptor superfamily member 6, Apo-1 antigen, Apoptosis-mediating surface antigen FAS, FASLG receptor

2 Images
Sandwich ELISA - Rat Fas ELISA Kit (AB245706)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Fas ELISA Kit (AB245706)

Example of rat Fas standard curve in Sample Diluent NS. The Fas standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Rat Fas ELISA Kit (AB245706)
  • sELISA

Supplier Data

Sandwich ELISA - Rat Fas ELISA Kit (AB245706)

Interpolated concentrations of native Fas in rat unstimulated thymus tissue culture supernatant and rat spleen tissue culture stimulated with PMA and Ionomycin.

Interpolated concentrations of native Fas in rat unstimulated thymus tissue culture supernatant (3 days) and rat spleen tissue culture stimulated with PMA (50 ng/mL) and Ionomycin (500 ng/mL) for 3 days. The concentrations of Fas were measured in duplicates, interpolated from the Fas standard curves and corrected for sample dilution. Undiluted samples are as follows : thymus tissue supernatant 50% and spleen tissue supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fas concentration was determined to be 5,444 pg/mL in neat thymus tissue culture supernatant and 791.4 pg/mL in spleen tissue culture supernatant. Unstimulated spleen supernatant was not tested.

关键信息

检测方法

Colorimetric

样品类型

Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

反应种属

Rat

检测类型

Sandwich

灵敏度

= 26.7 pg/mL

范围

156.3 - 10000 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

产品详情

Rat Fas ELISA Kit (ab245706) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Fas protein in cell culture media, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Fas with 26.7 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

精确度

[ { "reproducibilityType": "Inter", "sample": "Protein", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.8" }, { "reproducibilityType": "Intra", "sample": "Protein", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" } ]

回收率

[ { "sample": "Serum", "range": "91 - 92 %", "average": "= 91" }, { "sample": "EDTA Plasma", "range": "94 - 97 %", "average": "= 95" }, { "sample": "Cell culture media", "range": "107 - 110 %", "average": "= 109" }, { "sample": "Heparin Plasma", "range": "96 - 99 %", "average": "= 97" }, { "sample": "Citrate plasma", "range": "89 - 92 %", "average": "= 91" } ]

规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab245706-1x96Tests", "assetComponentDetails": [ { "size":"1 x 600 µL", "name":"10X Rat Fas Detector Antibody", "number":"AB245706-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB245706-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB245706-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB245706-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB245706-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB245706-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB245706-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB245706-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat Fas Capture Antibody", "number":"AB245706-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rat Fas Lyophilized Recombinant Protein", "number":"AB245706-CMP04", "productcode":"" } ] } } }

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C

产品实验方案

靶点信息

Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase CASP8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs CASP8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both (By similarity).
See full target information Fas
websiteProtocolBooklet
en

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