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大鼠BNP 45 ELISA试剂盒(ab108816)

Reviews (1)Q&A (10)References (27)
Typical Standard Curve
  • Sandwich ELISA - BNP 45 Rat ELISA Kit (ab108816)

Key features and details

  • Sensitivity: 7.9 ng/ml
  • Range: 15.625 pg/ml - 1000 pg/ml
  • Sample type: Cell Lysate, Plasma, Serum, Tissue
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Rat

概述

  • 产品名称

    大鼠BNP 45 ELISA试剂盒

  • 检测方法

    Colorimetric

  • 精确度

    批次内
    样品 n Mean SD CV%
    Overall 5.5%
    批次间
    样品 n Mean SD CV%
    Overall 10.4%

  • 样品类型

    Serum, Plasma, Tissue, Cell Lysate

  • 检测类型

    Sandwich (quantitative)

  • 灵敏度

    = 7.9 ng/ml

  • 范围

    15.625 pg/ml - 1000 pg/ml

  • 实验步骤

    Multiple steps standard assay

  • 种属反应性

    与反应: Rat

  • 产品概述

    Abcam’s BNP 45 rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of BNP 45 in plasma, serum, cell lyate and tissue samples.


    A BNP 45 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a BNP 45 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of BNP 45 captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • 说明

    There is significant cross-reactivity between BNP32 and BNP45.

  • 经测试应用

    适用于: Sandwich ELISAmore details

  • 平台

    Microplate

性能

  • 存放说明

    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    70X Biotinylated Rat BNP 45 Antibody 1 x 90µl
    BNP 45 Microplate (12 x 8 well strips) 1 unit
    BNP 45 Standard (Lyophilized) 1 vial
    Chromogen Substrate 1 x 7ml
    Sealing Tapes 3 units
    Stop Solution 1 x 11ml

  • 研究领域

  • 功能

    Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.

  • 组织特异性

    Brain and also in atria, but at much lower levels than ANP.

  • 序列相似性

    Belongs to the natriuretic peptide family.

  • 细胞定位

    Secreted.
  • Information by UniProt

  • 别名

    • ANFB_HUMAN
    • BNP(1-32)
    • BNP(5-29)
    • BNP-32
    • BNP-45
    • Gamma-brain natriuretic peptide
    • nppb
    see all

  • 数据库链接

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab108816于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Sandwich ELISA
    Use at an assay dependent concentration.
    说明
    Sandwich ELISA
    Use at an assay dependent concentration.

    图片

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab108816

    • Sandwich ELISA - BNP 45 Rat ELISA Kit (ab108816)
      Sandwich ELISA - BNP 45 Rat ELISA Kit (ab108816)
      Rat BNP45 measured in biological fluids showing quantity (pg) per mL of tested sample

    文献 (27)

    发表研究结果有使用 ab108816？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab108816 被引用在 27 文献中.

    • Haesen S  et al. Pyridoxamine Limits Cardiac Dysfunction in a Rat Model of Doxorubicin-Induced Cardiotoxicity. Antioxidants (Basel) 13:N/A (2024). PubMed: 38247537
    • Yin RT  et al. Open thoracic surgical implantation of cardiac pacemakers in rats. Nat Protoc 18:374-395 (2023). PubMed: 36411351
    • Yoshida K  et al. Vagal nerve stimulation preserves right ventricular function in a rat model of right ventricular pressure overload. Pulm Circ 12:e12154 (2022). PubMed: 36419525
    • Xiong Y  et al. Regulation of SARS CoV-2 host factors in the kidney and heart in rats with 5/6 nephrectomy-effects of salt, ARB, DPP4 inhibitor and SGLT2 blocker. BMC Nephrol 23:117 (2022). PubMed: 35331159
    • Wang Z  et al. NBP Relieves Cardiac Injury and Reduce Oxidative Stress and Cell Apoptosis in Heart Failure Mice by Activating Nrf2/HO-1/Ca2+-SERCA2a Axis. Evid Based Complement Alternat Med 2022:7464893 (2022). PubMed: 36452141
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 11 Abreviews or Q&A

    Rat BNP 45 ELISA Kit

     Good Good 4/5 (Ease of Use)
    Abreviews
    Rat BNP 45 ELISA Kit
    The ELISA Kit is easy to use and works well.
    Rat plasma was used with 10 times dilution.(For reference)
    Antibody incubation time is a bit long.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jul 10 2017

    Question

    Hello tech support team! How are you?



    I have a customer enquiring about finding a similar antibody to AB108816 that would react with Human instead of Rat.



    Could you please advise us whether there’s such product reacting with Human or that the above product AB108816 can also be used in experimenting with Human?



    Really appreciate your help!



    Looking forward to your response soon!



    Have a nice day!



    Kind regards,

    Read More
    Answer

    Thank you for your enquiry.

    Currently, this is the only BNP 45 ELISA kit (ab108816) which recognizes rat BNP 45. We sell primary antibodies which detect human BNP:

    ab20984,

    ab19645,

    ab19646,

    ab47699,

    ab66240

    I hope this helps and if I can assist further, please do not hesitate to contact me.

    Read More

    Question

    Is there a chance that this kit will cross react with rabbit samples?

    Read More
    Answer

    Our samples showed around 40% cross-reactivity for rabbit protein.

    Read More

    Question

    Young rat blood samples.
    Is the kit ab108815 and/or ab108816 suitable for plasma obtained from blood collected using heparin tubes, centrifuged and stored at -80ºC?
    Also, what is the difference between the BNP-32 and BNP-45 ?

    Read More
    Answer

    Merci de nous avoir contactés.

    Les collaborateurs du laboratoire où les kits ELISA ab108815 et ab108816 ont été développés m'ont informé que:

    1) Les deux kits peuvent en effet être utilisés sur plasma hépariné.

    2) BNP32 et BNP45 correspondent à 2 formes de la BNP. Le croisement entre les 2 kits est d'environ 60%. D'après mes collaborateurs, il est parfois décrit que la BNP45 est plus abondante que la BNP32, et parfois il est décrit que c'est l'inverse. Le choix sur la forme de la BNP à mesurer est donc laissé à l'expérimentateur.

    J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

    Read More

    Question

    Is it ok to keep on feeding the rats prior to the experiments?

    Read More
    Answer

    Vielen Dank für Ihren Anruf.

    Wenn das Futter keinen Einfluss auf die Produktion dieser Peptide hat (wie durch zB salzreiche Nahrung), sehe ich keinen Grund, warum die Ratten fasten sollten.

    Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

    Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
    https://www.abcam.com/abreviews

    Read More

    Question

    I received the kit ab108816 (BNP 45 Rat ELISA kit) and it was improperly stored in the freezer for two days, it can change its performance? I will use it on Tuesday.

    Thank you

    Read More
    Answer

    Thank you for contacting us.

    The best way to test the viability is using the kit. The kits should be fine at -20C so please use the kit without any hesitation.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question


    The answers followed below:
    1) The sample collected to quantify the concentration of BNP was the
    blood plasma of Wistar rats.
    After the samples
    had been collected it was transferred to a test tube suitable for the
    volume collected and
    centrifuged for 10 min at 2000 rcf and 4C. After centrifugation,
    plasma was divided
    into 4 aliquots of 200ul in ependorfs of 1.5 mL. After having been
    aliquoted plasma was
    frozen in liquid nitrogen and stored in a freezer at-80C until the day
    that one of these
    aliquots were unfreezed to perform the ELISA.

    2) In this study two methods are being used to quantify the BNP: ELISA
    and qRT-PCR.
    The quantification is being processed in two distinct periods, two and
    five months after
    the animals have completed treatment to which they have undergone. The
    results of the
    first ELISA were consistent with the results of qRT-PCR, meaning that
    the average of
    the analyzed groups had the same place in both methods, for example:
    group A had the
    second highest average, the group the higher average B and group C at
    the third highest
    average, all in both the ELISA and the qRT-PCR. But this did not happen with the
    second kit. In the second ELISA, the A group obtained the highest
    average in the first
    ELISA, but the second highest average in qRT-PCR and group B had the
    second highest
    average ELISA, but the highest average qRT-PCR, and group C was
    remained the third
    highest average in both the ELISA and the qRT-PCR. But it is not only
    this, compared
    to the first kit, the second kit had twice the samples with 15% CV
    above (the first of the
    samples was 6.06% with CVs higher than 15% and the second 12%). The
    calculation of
    intra-assay reproducibility of the first was 4.2% and 6.6% of the
    second. As all samples
    were collected under the same conditions, the first and second kit
    being from different
    batches and the second kit submitting the fault of does not have the
    information of the
    quantity of pattern provided, although not having problems with the
    standard curve,
    these facts lead me to suspect that may have occurred other problems
    in the production
    of the kit beyond of not contain the addition amount of the standard provided.

    3) To perform both kits, all the step by step information of the same
    datasheet were followed. None of the kits used diluted samples.

    Read More
    Answer

    Thank you for your reply and the additional information.

    We have the following PO on file: xxx.The order was from xxx and the lot number matches what you had sent, thus I think this is the correct order.


    I have now arranged the free of charge replacement.

    Please accept my sincere apologies for the problems with the second kit.
    Please do not hesitate to contact me if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    Our customer complained about a Abcam kit that she bought.
    She bought two BNP 45 kit for rat (ab108816). The first one, lote
    GR53511-2 , worked well. The second kit was the problem, lote
    GR53511-4. First of all, there was missing the standard value at the
    kit. After the assay, the researcher showed that more than half of
    her samples did not work. She said that at the first kit all samples
    worked. The samples used on the first kit were extracted on the same
    way as the samples used on the second kit. She would like to receive a
    new kit to test her samples again.

    Read More
    Answer

    Thank you for contacting us.

    I am very sorry to hear about the problems your customer had to face because of the second kit. I sincerely apologize about that! Yes, I'd be happy to arrange for a free of charge replacement.


    Could you please let me know your order number or PO number with us for the second kit?

    Also, for our internal record, I would like to receive a couple more details from the customer:
    1) What type of samples were used and how were they prepared?
    2) What do the data look like? In which way were the data incorrect?
    3) Was the protocol followed step by step or were any deviations included?


    I look forward to hear back from you and to assist you in resolving this case.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    Buenos dias,
    Adjunto una foto de dos viales de estandar del kit recibidos en dos pedidos diferentes.
    En el segundo pedido no se especificaba la cantidad de estandar contenida.
    Podrian por favor indicarnos de que cantidad se trata?
    Muchas gracias.

    Read More
    Answer

    Gracias por contactarnos y alertarnos de este fallo.



    Lamento mucho que el vial de estándar del nuevo lote enviado no indicara ningún dato acerca de la cantidad contenida. Sin duda se trata de un error que estamos tratando de resolver lo antes posible para que las nuevas etiquetas, así como la datasheet contengan la información necesaria.

    He comprobado que el vial recibido del lote XXXX contiene la misma cantidad de estándar que el anterior (4ng).

    También aprovecho para recordarte que todos nuestros productos están cubiertos por la garantía Abpromise de 6 meses, y en caso de no funcionar como se especifica en su datasheet, se os reemplazará el producto de forma gratuita o se reembolsará el importe del mismo.

    Espero haberte ayudado. Para cualquier otra consulta o sugerencia, por favor no dudes en volvernos a contactar.

    Read More

    Question

    Could you please clarify me for how long should I incubate the chromogen substrate? 10 min or 15 min? -> protocol, page 4: Add 50 μl of Chromogen Substrate to each well. Incubate 10 minutes or till the optimal blue colour density develops. -> protocol, page 10: Add 50 μl of Chromogen Substrate per well and incubate for about 15 minutes or till the optimal blue color density develops. Also, for plasma samples is it necessary to dilute them? Thank you in advance. Regards,

    Read More
    Answer

    Thank you for your enquiry and your interest.

    As the Protocol booklet indicates we would suggest adding 50 μl of Chromogen Substrate per well and incubate for about 15 minutes or till the optimal blue colour density develops. The optimal time points and the dilution(s) needs to be optimized by the end user since the BNP 45 levels depends on the samples.

    I hope this helps and if I can assist further, please do not hesitate to contact me.

    Read More

    1-10 of 11 Abreviews or Q&A

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